A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues.
A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues.
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DOI：
10.1016/s0021-9258(17)41524-9
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发表时间：
1945-11
期刊：
影响因子：
--
通讯作者：
G. Schmidt;S. J. Thannhauser
中科院分区：
文献类型：
--
作者：
G. Schmidt;S. J. Thannhauser
用冷的三氯乙酸提取切碎的组织以除去酸溶性P，用乙醇-乙醚和甲醇-氯仿提取以除去类脂P。残留物用N-氢氧化钾在37 ℃下消化至少15小时。将核糖核酸分解成组成核糖核酸的核苷酸，并将无机磷从磷蛋白中分离出来。总P估计在碱性消化物的一部分中。这给出了核糖核酸P加脱氧核糖核酸P加磷蛋白P的量度。另一部分碱性消化物用HCl和三氯乙酸处理以沉淀脱氧核糖核酸。在上清液中测定总P和无机P。它们之间的差异给出了核糖核酸P的量，而无机P是磷蛋白P的量度。脱氧核糖核酸P由碱性消化物中的总P和消化物酸化后上清液中的总P之间的差异给出。J. N.戴维森.
The finely minced tissue is extracted with cold trichloro-acetic acid to remove acid-soluble P and with ethyl alcohol-ether and methyl alcoholchloroform to remove lipoid P. The residue is digested withNpotassium hydroxide for at least 15 hr. at 37° C. to break down ribonucleic acid into its constituent nuoleotides and to split inorganic P from phosphoproteins. Total P is estimated in a portion of the alkaline digest. This gives a measure of ribonucleic acid P plus desoxyribonucleic acid P plus phosphoprotein P. Another portion of the alkaline digest is treated with HC1 and trichloroacetic acid to precipitate desoxyribonucleic acid. Total P and inorganic P are determined in the supernatant fluid. The difference between them gives the amount of ribonucleic acid P, while the inorganic P is a measure of the phosphoprotein P. The desoxyribonucleic acid P is given by the difference between the total P in the alkaline digest and the total P in the supernatant fluid after acidification of the digest.-J. N. Davidson.