Runx expression is mitogenic and mutually linked to Wnt activity in blastula-stage sea urchin embryos.
Runx expression is mitogenic and mutually linked to Wnt activity in blastula-stage sea urchin embryos.
复制标题
RUNX表达是有丝分裂的，并且与胚阶段海胆胚胎中的Wnt活性相互联系。
DOI：
10.1371/journal.pone.0003770
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Coffman, James A.
中科院分区：
文献类型：
--
作者：
Robertson, Anthony J.;Coluccio, Alison;Knowlton, Peter;Dickey-Sims, Carrie;Coffman, James A.
The Runt homology domain (Runx) defines a metazoan family of sequence-specific transcriptional regulatory proteins that are critical for animal development and causally associated with a variety of mammalian cancers. The sea urchin Runx gene SpRunt-1 is expressed throughout the blastula stage embryo, and is required globally during embryogenesis for cell survival and differentiation. Depletion of SpRunt-1 by morpholino antisense-mediated knockdown causes a blastula stage deficit in cell proliferation, as shown by bromodeoxyuridine (BrdU) incorporation and direct cell counts. Reverse transcription coupled polymerase chain reaction (RT-PCR) studies show that the cell proliferation deficit is presaged by a deficit in the expression of several zygotic wnt genes, including wnt8, a key regulator of endomesoderm development. In addition, SpRunt-1-depleted blastulae underexpress cyclinD, an effector of mitogenic Wnt signaling. Blastula stage cell proliferation is also impeded by knockdown of either wnt8 or cyclinD. Chromatin immunoprecipitation (ChIP) indicates that Runx target sites within 5′ sequences flanking cyclinD, wnt6 and wnt8 are directly bound by SpRunt-1 protein at late blastula stage. Furthermore, experiments using a green fluorescent protein (GFP) reporter transgene show that the blastula-stage operation of a cis-regulatory module previously shown to be required for wnt8 expression (Minokawa et al., Dev. Biol. 288: 545–558, 2005) is dependent on its direct sequence-specific interaction with SpRunt-1. Finally, inhibitor studies and immunoblot analysis show that SpRunt-1 protein levels are negatively regulated by glycogen synthase kinase (GSK)-3. These results suggest that Runx expression and Wnt signaling are mutually linked in a feedback circuit that controls cell proliferation during development.
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影响因子：
4.8
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评估吗啉代反义靶向海胆 Runx 基因产生的发育表型。
影响因子：
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作者：
Coffman, James A.;Dickey-Sims, Carrie;Haug, Jeffrey S.;McCarthy, John J.;Robertson, Anthony J.
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