Functional pathway characterized by gene expression analysis of supraclavicular lymph node metastasis-positive breast cancer
Functional pathway characterized by gene expression analysis of supraclavicular lymph node metastasis-positive breast cancer
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DOI：
10.1007/s10038-007-0111-z
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发表时间：
2007-03-01
影响因子：
3.5
通讯作者：
Miki, Yoshio
中科院分区：
文献类型：
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作者：
Oishi, Yoko;Nagasaki, Koichi;Miki, Yoshio
乳腺癌锁骨上淋巴结转移患者预后一般较差，但也有部分患者存活时间较长。因此，预测预后的能力对于选择合适的治疗方法治疗锁骨上淋巴结转移的乳腺癌患者是很重要的。在这项研究中，我们试图通过分析cDNA微阵列来确定决定锁骨上淋巴结转移乳腺癌患者预后的功能途径。31例锁骨上淋巴结转移无远处转移的乳腺癌患者纳入研究队列；这些患者根据预后分为三组：不良、中等和良好。我们利用6个基因（DVL1、VDAC2、BIRC5、Stathmin1、PARP1和RAD21）构建了两条功能通路，即Wnt信号通路和线粒体凋亡通路，这些基因在预后良好组和预后不良组中表达不同。我们的研究结果表明，这两条功能通路可能在决定乳腺癌锁骨上淋巴结转移患者的预后中起重要作用。我们还确定Stathmin1基因产物的免疫组织染色是预测锁骨上淋巴结转移的乳腺癌患者预后的潜在工具。
The outcome of breast cancer patients with supraclavicular lymph node metastasis is generally poor, but some patients do survive for a long time. Consequently, the ability to predict the outcome is important in terms of choosing the appropriate therapy for breast cancer patients with supraclavicular lymph node metastasis. In this study, we attempted to identify functional pathways that determine the outcome of breast cancer patients with supraclavicular lymph node metastasis by profiling cDNA microarrays. Thirty-one breast cancer patients with supraclavicular lymph node metastasis without distant metastasis comprised the study cohort; these were divided into three groups based on prognosis - poor, intermediate, and good. Two functional pathways, the Wnt signaling pathway and the mitochondrial apoptosis pathway, were constructed using six genes (DVL1, VDAC2, BIRC5, Stathmin1, PARP1, and RAD21) that were differently expressed between the good and poor outcome groups. Our results indicate that these two functional pathways may play an important role in determining the outcome of breast cancer patients with supraclavicular lymph node metastasis. We also determined that immunohistostaining for the Stathmin1 gene product is a potential tool for predicting the outcome of breast cancer patients with supraclavicular lymph node metastasis.