RFLP mapping of QTLs for yield and related characters in rice (Oryza sativa L)
RFLP mapping of QTLs for yield and related characters in rice (Oryza sativa L)
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DOI：
10.1007/bf00224031
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发表时间：
1996-06-01
影响因子：
5.4
通讯作者：
Zheng, KL
中科院分区：
文献类型：
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作者：
Lin, HX;Qian, HR;Zheng, KL
以特三矮2号/CB和外引2号/CB两个籼/籼稻F-2群体为材料，利用RFLP图谱定位了水稻产量及相关性状的QTL。在特三矮2/CB中共检测到14个QTL区间，其中单株粒重3个，单株穗数2个，每穗粒数2个，每穗总小穗数1个，小穗结实率1个，千粒重3个，小穗密度1个，I为每穗一级分枝数。GWT的3个QTL分别位于第1、2和4染色体上，每条染色体上各有1个QTL。单基因加性效应范围为2.0~9.1g，在第4染色体RG143~RG214区间内检测到一个NP主效基因（np4），该基因对NP的解释率为26.1%。该基因座的父本等位基因负责减少每株植物的穗数（每株植物3穗）。在外引2号/CB群体中，检测到12个QTL区间，其中3个为GWT，2个为NP、TNS、TGWT和SD，1个为SF。3个GWT QTL分别位于第1、4和5染色体上。单位点加性效应在6.7~8.8g之间，显性效应在1.7-11.5g之间。比较和讨论了两个具有共同父本的群体的QTL定位。
Quantitative trial loci (QTLs) for yield and related traits in rice were mapped based on RFLP maps from two indica/indica F-2 populations, Tesanai 2/CB and Waiyin 2/CB. In Tesanai 2/CB 14 intervals carrying QTLs for eight traits were detected, including 3 for grain weight per plant (GWT), 2 for number of panicles per plant (NP), 2 for number of grains per panicle (NG), 1 for total number of spikelets per panicle (TNS), 1 for spikelet fertility (SF) 3 for 1000-grain weight (TGWT), 1 for spikelet density (SD), and I for number of first branches per main panicle. The 3 QTLs for GWT were located on chromosomes 1, 2, and 4, with 1 in each chromosome. The additive effect of the single locus ranged from 2.0 g to 9.1 g, A major gene (np4) for NP was detected on chromosome 4 within the interval of RG143-RG214, about 4 cM for RG143, and this locus explained 26.1% of the observed phenotypic variance for NP. The paternal allele of this locus was responsible for reduced panicles per plant (3 panicles per plant). In another population, Waiyin 2/CB, 12 intervals containing QTLs for six of the above-mentioned traits were detected, including 3 for GWT, 2 for each of NP, TNS, TGWT and SD, 1 for SF. Three QTLs for GWT were located on chromosome 1, 4, and 5, respectively. The additive effect of the single locus for GWT ranged from 6.7 g to 8.8 g, while the dominance effect was 1.7-11.5 g. QTL mapping in two populations with a common male parent is compared and discussed.