Pattern analysis of 532-and 1,064-nm picosecond-domain laserinduced immediate tissue reactions in ex vivo pigmented micropig skin
Pattern analysis of 532-and 1,064-nm picosecond-domain laserinduced immediate tissue reactions in ex vivo pigmented micropig skin
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DOI：
10.1038/s41598-019-41021-7
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发表时间：
2019-03-12
影响因子：
4.6
通讯作者：
Cho, Sung Bin
中科院分区：
文献类型：
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作者：
Lee, Hee Chul;Childs, James;Cho, Sung Bin
利用分束器或微透镜阵列(MLA)，来自皮秒激光的光脉冲可以作为单束、平顶光束或分束传输到皮肤。在这项研究中，使用单一平顶光束和波长分别为532 nm和1,064 nm的MLA型光束，对受基因调控的体外着色小型猪皮肤进行了皮秒激光治疗。治疗结束后立即取皮肤标本进行光镜分析。与未经处理的对照组相比，波长为532 nm、注量为0.05-J/cm(2)的单一平顶光束处理减少了表皮角质形成细胞和黑素细胞中的黑色素。此外，0.1J/cm(2)和1.3J/cm(2)的532 nm激光诱导所有表皮层内的角质形成细胞和黑素细胞出现明显的空泡化。波长为1,064 nm、能量密度为0.18 J/cm(2)的单束平顶皮秒激光也减少了角质形成细胞和黑素细胞中的黑色素。在1,064 nm和1.4J/cm(2)和2.8J/cm(2)的照射剂量下，角质形成细胞和黑素细胞的空泡化程度增加。同时，532 nm和1064 nm MLA类型的皮秒激光治疗在表皮和真皮中引发了激光诱导的微空泡化的分割区域。其中，组织反应的大小和程度因波长、通量和微透镜与皮肤之间的距离而不同。
Optical pulses from picosecond lasers can be delivered to the skin as single, flat-top beams or fractionated beams using a beam splitter or microlens array (MLA). In this study, picosecond neodymium: yttrium aluminum garnet laser treatment using a single flat-top beam and an MLA-type beam at the wavelengths of 532 nm and 1,064 nm were delivered on ex vivo genotype-regulated, pigmented micropig skin. Skin specimens were obtained immediately after treatment and microscopically analyzed. Single flat-top beam treatment at a wavelength of 532 nm and a fluence of 0.05-J/cm(2) reduced melanin pigments in epidermal keratinocytes and melanocytes, compared to untreated controls. Additionally, 0.1 J/cm(2)-and 1.3 J/cm(2)-fluenced laser treatment at 532 nm elicited noticeable vacuolation of keratinocytes and melanocytes within all epidermal layers. Single flat-top beam picosecond laser treatment at a wavelength of 1,064 nm and a fluence of 0.18 J/cm(2) also reduced melanin pigments in keratinocytes and melanocytes. Treatment at 1,064-nm and fluences of 1.4 J/cm(2) and 2.8 J/cm(2) generated increasing degrees of vacuolated keratinocytes and melanocytes. Meanwhile, 532-and 1,064-nm MLA-type, picosecond laser treatment elicited fractionated zones of laser-induced micro-vacuolization in the epidermis and dermis. Therein, the sizes and degrees of tissue reactions differed according to wavelength, fluence, and distance between the microlens and skin.