Microglial cells in culture express a prominent glutathione system for the defense against reactive oxygen species
Microglial cells in culture express a prominent glutathione system for the defense against reactive oxygen species
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DOI：
10.1159/000017464
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发表时间：
2000-09-01
影响因子：
2.9
通讯作者：
Dringen, R
中科院分区：
文献类型：
--
作者：
Hirrlinger, J;Gutterer, JM;Dringen, R
为了获得关于小胶质细胞的谷胱甘肽代谢的信息，测定了来自大鼠脑的富含小胶质细胞的培养物的谷胱甘肽含量和参与防御过氧化物的酶的活性。这些培养物含有约90%的小胶质细胞，这是通过胶质标志物的免疫细胞化学染色、细胞的吞噬活性以及用佛波酯刺激细胞后超氧化物的产生来确定的。对于这些培养物，测定谷胱甘肽含量为41.2 +/- 11.2 nmol/mg蛋白质，谷胱甘肽还原酶的比活性为15.2 +/- 3.2 nmol/（min x mg蛋白质）。这些值显著高于星形胶质细胞或神经元培养物中发现的值。此外，在68.7 +/- 23.5 nmol/（min x mg蛋白质）时，小胶质细胞培养物中谷胱甘肽过氧化物酶的比活性比培养的神经元高78%。比过氧化氢酶活性的小胶质细胞培养物是小于40%的星形胶质细胞或神经元培养物。小胶质细胞培养物仅含有少量的氧化型谷胱甘肽。然而，在应用氧化应激与过氧化氢或与超氧化物产生次黄嘌呤/黄嘌呤氧化酶系统的小胶质细胞培养物孵育，细胞谷胱甘肽被迅速氧化。这些结果表明，小胶质细胞具有突出的谷胱甘肽系统，这可能反映了当由这些或周围脑细胞产生时针对活性氧的自我保护的必要性。版权所有（C）2000 S. Karger AG，巴塞尔。
To obtain information on the glutathione metabolism of microglial cells, the content of glutathione and activities of enzymes involved in the defense against peroxides were determined for microglia-rich cultures from rat brain. These cultures contain approximately 90% microglia cells as determined by immunocytochemical staining for glial markers, by the phagocytosis activity of the cells and by the production of superoxide after stimulation of the cells with phorbolester. For these cultures, a glutathione content of 41.2 +/- 11.2 nmol/mg protein and a specific activity of glutathione reductase of 15.2 +/- 3.2 nmol/(min x mg protein) were determined. These values are significantly higher than those found for astroglial or neuronal cultures. In addition, with 68.7 +/- 23.5 nmol/(min x mg protein), the specific activity of glutathione peroxidase in microglial cultures was 78% higher than in cultured neurons. The specific catalase activity of microglial cultures was less than 40% that of astroglial or neuronal cultures. Microglial cultures contain only marginal amounts of oxidized glutathione. However, on application of oxidative stress by incubation of microglial cultures with hydrogen peroxide or with the superoxide-producing hypoxanthine/xanthine oxidase system, cellular glutathione was rapid ty oxidized. These results demonstrate that microglial cells have a prominent glutathione system, which is likely to reflect the necessity for self-protection against reactive oxygen species when produced by these or surrounding brain cells. Copyright (C) 2000 S. Karger AG, Basel.