Absence of Early Neuronal Death in the Olivocochlear System Following Acoustic Overstimulation
Absence of Early Neuronal Death in the Olivocochlear System Following Acoustic Overstimulation
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声学过度刺激后橄榄耳蜗系统不存在早期神经元死亡
DOI：
10.1002/ar.23277
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
S. Wolpert
中科院分区：
文献类型：
--
作者：
S. Reuss;Christina Closhen;R. Riemann;Mirko Jaumann;M. Knipper;L. Rüttiger;S. Wolpert
本研究旨在探讨噪声损伤对橄榄耳蜗（OC）神经元的影响。将麻醉大鼠暴露于120 dB声压级的10 kHz纯音持续2小时。治疗效果通过听性脑干反应和畸变产物耳声发射的记录来验证。3或8天后，大鼠接受单侧注射的逆行神经元示踪剂荧光金（FG）的水溶液到鼓阶，以确定OC神经元（OCN）。FG注射后5天，将脑灌注固定，并切割脑干切片并分析FG标记的神经元。我们发现，在两组中，OCN的数量与对照组相似。第二组切片与胆碱乙酰转移酶（负责乙酰胆碱合成的酶）的抗体一起孵育，显示了脑干的胆碱能神经元，然而，没有表明组间的差异。我们的研究是第一个调查噪音创伤对已识别OCN影响的研究，结果显示，在创伤后2周内，已识别OCN和胆碱乙酰转移酶免疫荧光均未发生明显变化。此时，听性脑干反应和畸变产物耳声发射测量显示严重的听力损失迹象。噪声性听力损失的发生机制与OCN的变性无关。Anat Rec，299：103-110，2016.© 2015 Wiley Periodicals，Inc.
This study was conducted to examine possible effects of noise trauma on olivocochlear (OC) neurons. Anesthetized rats were exposed to a continuous 10 kHz pure tone at 120 dB sound pressure level for 2 hrs. The effects of treatment were verified by recordings of auditory brainstem response and distortion product otoacoustic emission. Three or 8 days after acoustic trauma, rats received unilateral injections of an aqueous solution of the retrograde neuronal tracer Fluorogold (FG) into the scala tympani to identify OC neurons (OCN). Five days after FG injection, brains were perfusion‐fixed, and brainstem sections were cut and analyzed with respect to FG‐labeled neurons. We found that, in both groups, numbers of OCN were similar to that of controls. The incubation of a second set of sections with antibodies against choline‐acetyltransferase (the enzyme responsible for acetylcholine synthesis) showed the cholinergic neurons of the brainstem, however, without suggesting differences between groups. Our study, the first to investigate noise trauma effects on identified OCN, revealed that no visible alterations occurred in 2 weeks following trauma, neither in identified OCN nor in choline‐acetyltransferase‐immunofluorescence. At this time, auditory brainstem response and distortion product otoacoustic emission measurements showed severe signs of hearing loss. The mechanisms leading to hearing loss upon noise trauma apparently do not involve degeneration of OCN. Anat Rec, 299:103–110, 2016. © 2015 Wiley Periodicals, Inc.
影响因子：
5.1
作者：
W. Singer;A. Zuccotti;Mirko Jaumann;S. C. Lee;R. Panford-Walsh;Hao Xiong;U. Zimmermann;Christoph Franz;Hyun-Soon Geisler;I. Köpschall;K. Rohbock;Ksenya Varakina;S. Verpoorten;T. Reinbothe;T. Schimmang;L. Rüttiger;M. Knipper
通讯作者：
W. Singer;A. Zuccotti;Mirko Jaumann;S. C. Lee;R. Panford-Walsh;Hao Xiong;U. Zimmermann;Christoph Franz;Hyun-Soon Geisler;I. Köpschall;K. Rohbock;Ksenya Varakina;S. Verpoorten;T. Reinbothe;T. Schimmang;L. Rüttiger;M. Knipper
影响因子：
2.5
作者：
KAWASE, T;DELGUTTE, B;LIBERMAN, MC
通讯作者：
LIBERMAN, MC
耳鸣的神经生理机制。
影响因子：
1.2
作者：
Kaltenbach,JA
通讯作者：
Kaltenbach,JA