Nitric oxide synthases in the human cervix at term pregnancy and effects of nitric oxide on cervical smooth muscle contractility
Nitric oxide synthases in the human cervix at term pregnancy and effects of nitric oxide on cervical smooth muscle contractility
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DOI：
10.1067/mob.2000.105901
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发表时间：
2000-09-01
影响因子：
9.8
通讯作者：
Norström, A
中科院分区：
文献类型：
--
作者：
Ekerhovd, E;Brännström, M;Norström, A
目的：本研究的目的是确定足月妊娠时宫颈中是否存在一氧化氮生成系统，并研究一氧化氮对宫颈条带收缩的影响。研究设计：组织标本取自分娩后和择期剖宫产妇女的子宫。采用免疫组织化学技术和免疫印迹技术鉴定一氧化氮合酶的同工异构体。用硝酸甘油或精胺nooate [(Z)-1-（N-[3-氨基丙基]-N-[4-（3-氨基丙基-氨）丁基]-氨基）-重氮-1-ium-1,2-二酸盐]加入器官浴中，研究了一氧化氮对宫颈收缩力的影响。结果：免疫组化检查显示内皮和诱导型一氧化氮合酶阳性。两种一氧化氮合酶异构体均可通过免疫印迹法清晰检测到。施用硝酸甘油或精胺NONOate时，观察到明显抑制收缩活性(10（-7)-10(-5)mol/L）。结论：足月子宫颈存在内源性一氧化氮系统，该组织对一氧化氮有反应，导致宫颈肌肉松弛。
OBJECTIVE: The purpose of the study was to determine whether a nitric oxide-generating system exists in the uterine cervix at term pregnancy and to study the effects of nitric oxide on contracting cervical strips.STUDY DESIGN: Tissue specimens were obtained from the cervices of women after deliveries and at elective cesarean deliveries. Immunohistochemical techniques and immunoblotting were used to identify isoforms of nitric oxide synthase. The effects of nitric oxide on cervical contractility were examined by the addition of nitroglycerin or spermine NONOate [(Z)-1-(N-[3-aminopropyl]-N-[4-(3-aminopropyl-ammonio)butyl]-amino)-diazen-1-ium-1,2-diolate] to organ baths.RESULTS: Immunohistochemical examination demonstrated positive staining for both endothelial and inducible nitric oxide synthase. Both isoforms of nitric oxide synthase were clearly detectable by immunoblotting. Significant inhibition of contractile activity (10(-7)-10(-5) mol/L) was observed when nitroglycerin or spermine NONOate was administered.CONCLUSION: An endogenous nitric oxide system is present in the uterine cervix at term, and this tissue is responsive to nitric oxide, which causes relaxation of the cervical muscle.