Noninvasive assessment of the developing Xenopus cardiovascular system using optical coherence tomography
Noninvasive assessment of the developing Xenopus cardiovascular system using optical coherence tomography
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DOI：
10.1073/pnas.94.9.4256
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发表时间：
1997-04-29
影响因子：
11.1
通讯作者：
Fujimoto, JG
中科院分区：
文献类型：
--
作者：
Boppart, SA;Tearney, GJ;Fujimoto, JG
研究正常和异常的心脏发育往往受到无法评估完整生物体内心血管功能的限制。本工作引入光学相干层析成像(OCT)，这是一种基于背向散射红外光测量的微米级非侵入性成像方法，用于高分辨率评估非洲爪哇发育中的心血管系统的结构和功能。显微结构细节，如脑室大小和室壁位置，用16微米分辨率的OCT描绘，并与组织学相关联。心血管系统的三维显示也是通过每隔25微米的重复横断面成像实现的。除了结构信息，OCT还提供了高速的活体轴向测距和成像，从而可以评估定量的动态活动，如心室射血分数。OCT对动态评估的敏感性被证明是通过改变心脏功能和大小的变力剂，光学相干断层扫描是一种有吸引力的新技术，因为它具有高分辨率，能够通过不透明的结构成像，并且其廉价的便携式设计，在体内和体外成像的分辨率接近组织病理学，不需要动物被处死。
Studies investigating normal and abnormal cardiac development are frequently limited by an inability to assess cardiovascular function within the intact organism, Tn this work, optical coherence tomography (OCT), a new method of micron-scale, noninvasive imaging based on the measurement of backscattered infrared light, was introduced for the high resolution assessment of structure and function in the developing Xenopus laevis cardiovascular system. Microstructural details, such as ventricular size and wall positions, were delineated with OCT at 16-mu m resolution and correlated with histology. Three-dimensional representation of the cardiovascular system also was achieved by repeated cross-sectional imaging at intervals of 25 mu m. In addition to structural information, OCT provides high speed in vivo axial ranging and imaging, allowing quantitative dynamic activity, such as ventricular ejection fraction, to be assessed. The sensitivity of OCT for dynamic assessment was demonstrated with an inotropic agent that altered cardiac function and dimensions, Optical coherence tomography is an attractive new technology for assessing cardiovascular development because of its high resolution, its ability to image through nontransparent structures, and its Inexpensive portable design, in vivo and in vitro imaging are performed at a resolution approaching that of histopathology without the need for animal killing.