Antimicrobial coating of spider silk to prevent bacterial attachment on silk surgical sutures
Antimicrobial coating of spider silk to prevent bacterial attachment on silk surgical sutures
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DOI：
10.1016/j.actbio.2019.09.004
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发表时间：
2019-11-01
影响因子：
9.7
通讯作者：
Reis, Rui L.
中科院分区：
文献类型：
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作者：
Franco, Albina R.;Fernandes, Emanuel M.;Reis, Rui L.
术后或其他医疗相关程序造成的微生物感染是一个严重的健康问题。目前，研究的重点是开发具有抗菌性能的新型无药物材料，以防止或最大限度地减少感染的风险。蜘蛛丝以其独特的生物力学性能和生物相容性而闻名。重组DNA技术可以用抗菌肽(AMP)对蜘蛛丝进行生物工程。因此，我们的目标是用AMP(6mer-HNP1)生物工程蜘蛛丝蛋白，作为商业丝线(Perma-Hand(R))的抗菌无药涂层，以减少细菌感染。Perma-Hand(R)缝线用6mer-HNP1浸渍涂层。使用人胎肺成纤维细胞(MRC5)进行的体外试验表明，涂层缝合线保持了细胞的活力，而且与红细胞(RBC)的接触也证明了血液相容性。此外，涂层显著抑制了生物被膜的附着和形成，与未涂层的Perma-Hand(R)缝合线相比，涂有6mer-HNP1的缝合线产生的耐甲氧西林金黄色葡萄球菌(MRSA)减少了1.5个对数，大肠杆菌(E.coli)减少了2个对数。Perma-Hand(R)缝合线的机械性能不受生物工程蜘蛛丝蛋白存在的影响。因此，本工作表明，使用蜘蛛丝无药涂层可以提高商业缝合线的抗菌性能。此外，可以开发一种新的无药物缝线来减少植入后感染。意义声明术后或其他与医疗相关的程序引起的微生物感染是一个严重的健康问题。开发具有抗菌性能的新型无药物材料是预防或最大限度减少感染风险的一种方法。蜘蛛丝以其独特的生物力学性能和生物相容性而闻名。重组DNA技术可以用抗菌肽(AMP)对蜘蛛丝进行生物工程。我们的目标是用AMP作为丝线缝合线的抗菌涂层，对蜘蛛丝蛋白进行生物工程处理。涂层表现出优异的抗菌性能，并保持了固有的机械特性。体外研究表明，涂层缝合线对细胞行为有积极作用。有了这种新的无药物生物工程蜘蛛丝涂层，就有可能开发出一种新的无药物缝合线，以减少植入后的感染。(C)2019 Acta Materialia Inc.由Elsevier Ltd.出版。保留所有权利。
Microbial infections from post-surgery or other medical-related procedure is a serious health problem. Nowadays, the research is focused on the development of new drug-free materials with antibacterial properties to prevent or minimize the risk of infections. Spider silk is known for its unique biomechanical properties allied with biocompatibility. Recombinant DNA technology allows to bioengineering spider silk with antimicrobial peptides (AMP). Thus, our goal was to bioengineered spider silk proteins with AMP (6mer-HNP1) as an antibacterial drug-free coating for commercial silk sutures (Perma-Hand (R)) for decreasing bacterial infections. Perma-Hand (R) sutures were coated with 6mer-HNP1 by dip coating. In vitro tests, using human fetal lung fibroblasts (MRC5), showed that coated sutures sustained cell viability, and also, the contact with red blood cells (RBCs) demonstrate blood compatibility. Also, the coatings inhibited significantly the adherence and formation of biofilm, where sutures coated with 6mer-HNP1 produced a 1.5 log reduction of Methicillin-Resistant Staphylococcus aureus (MRSA) and a 2 log reduction of Escherichia coli (E. coli) compared to the uncoated Perma-Hand (R) suture. The mechanical properties of Perma-Hand (R) sutures were not affected by the presence of bioengineered spider silk proteins. Thus, the present work demonstrated that using spider silk drug-free coatings it is possible to improve the antibacterial properties of the commercial sutures. Furthermore, a new class of drug-free sutures for reducing post-implantation infections can be developed.Statement of SignificanceMicrobial infections from post-surgery or other medical-related procedure is a serious health problem. Developing new drug-free materials with antibacterial properties is an approach to prevent or minimize the risk of infections. Spider silk is known for its unique biomechanical properties allied with biocompatibility. Recombinant DNA technology allow to bioengineering spider silk with antimicrobial peptides (AMP). Our goal is bioengineered spider silk proteins with AMP as an antibacterial coating for silk sutures. The coatings showed exceptional antibacterial properties and maintained intrinsic mechanical features. In vitro studies showed a positive effect of the coated sutures on the cell behavior. With this new drug-free bioengineered spider silk coating is possible to develop a new class of drug-free sutures for reducing post-implantation infections. (C) 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.