Genome-wide analysis of the light-harvesting chlorophyll a/b-binding gene family in apple (Malus domestica) and functional characterization of MdLhcb4.3, which confers tolerance to drought and osmotic stress.
Genome-wide analysis of the light-harvesting chlorophyll a/b-binding gene family in apple (Malus domestica) and functional characterization of MdLhcb4.3, which confers tolerance to drought and osmotic stress.
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DOI：
10.1016/j.plaphy.2020.06.022
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发表时间：
2020-06
期刊：
影响因子：
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通讯作者：
Shuang Zhao;Hanbing Gao;Jiawei Luo;Haibo Wang;Q. Dong;Yanpeng Wang;Kaiyan Yang;Ke Mao;
中科院分区：
文献类型：
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作者：
Shuang Zhao;Hanbing Gao;Jiawei Luo;Haibo Wang;Q. Dong;Yanpeng Wang;Kaiyan Yang;Ke Mao;
In higher plants, the light-harvesting chlorophylla/b-binding (Lhc) proteins function in multiple processes that are critical to plant growth, development, and abiotic stress response. However, theLhcgene family has not been well characterized in the important fruit crop, apple (Malus×domesticaBorkh.). In this study, we identified 27Lhcgenes in the apple genome. Phylogenetic analysis showed that theLhcgene family could be classified into three major subfamilies, each of whose members shared similar conserved motifs. Evolutionary analysis indicated that duplicatedMdLhcgenes were primarily under purifying selection.MdLhcs were expressed at varying levels in all tissues examined and showed different expression patterns under drought stress. The overexpression ofMdLhcb4.3in transgenic Arabidopsis and apple callus enhanced their tolerance to drought and osmotic stress. Taken together, these results demonstrate the important role of Lhc proteins in the regulation of plant resistance to drought and osmotic stress and provide valuable information for further study of Lhc functions in apple.
