Analysis of the association between polymorphisms in the vitamin D receptor (VDR) gene and dental caries in a Chinese population
Analysis of the association between polymorphisms in the vitamin D receptor (VDR) gene and dental caries in a Chinese population
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中国人群维生素D受体（VDR）基因多态性与龋齿的相关性分析
DOI：
10.4238/2015.september.28.15
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发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Guo, M. L.
中科院分区：
文献类型：
--
作者：
Hu, X. P.;Li, Z. Q.;Guo, M. L.
众所周知，环境对龋齿的发生和发展有影响；然而，几乎没有证据表明遗传成分会影响龋齿的易感性。本研究旨在探讨中国人群维生素D受体TaqI位点单核苷酸多态性与龋齿易感性的关系。本研究采用病例组（来自中国西北地区的264例龋齿患者）和对照组（来自同一地区的219例无龋齿或全身性疾病患者）进行病例对照研究。从研究参与者的外周静脉血中提取DNA；采用聚合酶链反应-限制性片段长度多态性测定TaqI位点基因型分布和等位基因频率。所得数据采用Hardy-Weinberg平衡和卡方检验进行统计学分析。以Tt基因型为参照，病例组Tt基因型出现频率（14.0%）显著高于对照组（4.3%）。与Tt基因型个体相比，杂合Tt基因型个体患龋齿的风险增加3.8倍。病例组t等位基因比例（7.0%）与对照组（2.1%）差异有统计学意义(P = 0.0003；OR = 3.592，置信区间95%(1.790-7.208)]。因此，我们的结果表明，等位基因“t”可能是决定中国西北地区个体龋齿易感性的遗传因素。
Environmental influences on the development and progression of dental caries are well known; however, there is little evidence of a genetic component imparting susceptibility to dental caries. The aim of this study was to investigate the relationship between a single nucleotide polymorphism in the vitamin D receptor TaqI locus and dental caries susceptibility in a Chinese population. This case-control study was conducted with a case group (264 patients with dental caries from northwestern China) and a control group (219 individuals without dental caries or systemic disease from the same area). DNA was extracted from the peripheral venous blood of the study participants; the distribution of TaqI locus genotypes and allele frequencies was determined via polymerase chain reaction-restriction fragment length polymorphism. The data obtained were statistically analyzed using the Hardy-Weinberg equilibrium and Chi-square test. The frequency of the Tt genotype in the case group (14.0%) was significantly higher than that in the control group (4.3%), as determined using the genotype TT as the reference. The risk of dental caries was increased 3.8-fold in individuals with the heterozygous Tt genotype compared to that in the individuals with the TT genotype. The proportion of the 't' allele in the case group (7.0%) and the control group (2.1%) was observed to be significantly different [P = 0.0003; OR = 3.592, confidence interval 95% (1.790-7.208)]. Our results therefore suggested that the allele 't' might be a genetic factor determining dental caries susceptibility in individuals from the northwest of China.