THE REPRODUCTIVE INCOMPATIBILITY SYSTEM IN DROSOPHILA-SIMULANS - DAPI-STAINING ANALYSIS OF THE WOLBACHIA SYMBIONTS IN SPERM CYSTS
THE REPRODUCTIVE INCOMPATIBILITY SYSTEM IN DROSOPHILA-SIMULANS - DAPI-STAINING ANALYSIS OF THE WOLBACHIA SYMBIONTS IN SPERM CYSTS
复制标题
DOI：
10.1006/jipa.1993.1044
复制
发表时间：
1993-05-01
影响因子：
3.4
通讯作者：
ROUSSET, F
中科院分区：
文献类型：
--
作者：
BRESSAC, C;ROUSSET, F
迄今为止用于检测沃尔巴克氏体（一种类似立克次体的果蝇内共生体，即，与测试菌株杂交、电子显微镜观察、胚胎的DAPI染色或通过PCR扩增特定序列是显著耗时的技术。用荧光染料DAPI染色精子囊肿是一种快速可靠的技术，可用于检测共生体和评估单个男性的感染水平。精子囊肿中含有丰富的Wolbachiacell，并在精子发生过程中在废物袋中消除。在所有已知的非共生菌株中，都没有这样的质量。在感染的菌株中，四环素处理导致精子包囊内共生体的消失。在老年男性中，观察到感染囊肿的比例下降。然而，仍然被感染的包囊中的共生体数量没有明显变化。在老年男性中观察到的生殖不相容性的减少可以用这个过程来解释，即，减少感染囊肿的频率。
Any hitherto available technique used to detect the presence of Wolbachia, a rickettsia-like endosymbiont ofDrosophila simulans,i.e., crossing with tester strains, electron microscope observations, DAPI-staining of embryo, or amplification of specific sequences by PCR are notably time-consuming techniques. Staining sperm cysts with the fluorochrome DAPI is here shown to be a fast and reliable technique to detect the symbiont and evaluate the level of infection of individual males.Wolbachiacells are abundant in sperm cysts and make up a mass which is eliminated in the waste bag during spermatogenesis. Such a mass was absent in all those strains known to be aposymbiotic. In infected strains, tetracycline treatment led to the disappearance of the symbionts within sperm cysts. In aging males, a decrease in the proportion of infected cysts were observed. There was however no apparent change in the number of symbionts in the cysts which remained infected. The reduction of reproductive incompatibility, which is observed in older males, may be explained by this process, i.e., a decrease in the frequency of infected cysts.