EXPRESSION OF C-FOS IN NIH3T3 CELLS IS VERY LOW BUT INDUCIBLE THROUGHOUT THE CELL-CYCLE
EXPRESSION OF C-FOS IN NIH3T3 CELLS IS VERY LOW BUT INDUCIBLE THROUGHOUT THE CELL-CYCLE
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DOI：
10.1002/j.1460-2075.1986.tb04269.x
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发表时间：
1986-04-01
期刊：
影响因子：
11.4
通讯作者：
MULLER, R
中科院分区：
文献类型：
--
作者：
BRAVO, R;BURCKHARDT, J;MULLER, R
已有研究表明，在生长因子刺激静止的NIH3T3小鼠成纤维细胞后，c-fos原癌基因可以迅速和瞬时地被诱导。为了探讨c-fos在生长控制机制中的可能作用，我们研究了它在NIH3T3细胞周期中的表达和诱导性。从这一分析中可以得出两个主要结论。首先，c-fos的表达不受细胞周期调控，在细胞周期的各个阶段几乎都检测不到。其次，处于细胞周期不同阶段的细胞(有丝分裂除外)与静止细胞一样对生长因子诱导的c-fos敏感。这些观察结果表明，c-fos基因的诱导在NIH3T3细胞的持续周期中不起作用，但它们与c-fos功能可能与诱导成纤维细胞的活性有关的假设完全一致。通过这样的功能，c-fos可以有助于将细胞移出静止状态。
It has previously been shown that the c-fos proto-oncogene is rapidly and transiently induced following growth factor stimulation of quiescent NIH3T3 mouse fibroblasts. To investigate a possible role of c-fos in growth control mechanisms we have studied its expression and inducibility during the NIH3T3 cell cycle. Two major concluuions can be drawn from this analysis. First, expression of c-fos is not cell cycle-regulated, and is barely detectable in all phases of the cycle. Second, cells at different stages of the cell cycle (except for mitosis) are as sensitive to c-fos induction by growth factors as quiescent cells. These observations suggest that induction of the c-fos gene does not play a role during the continuous cycling of NIH3T3 cells, but they are fully compatible with the hypothesis that a function of c-fos may be associated with the induction of competence in fibroblasts. Through such a function c-fos may contribute to moving cells out of the quiescent state.