Leishmania infantum and Neospora caninum simultaneous skin infection in a young dog in Italy
Leishmania infantum and Neospora caninum simultaneous skin infection in a young dog in Italy
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DOI：
10.1016/s0304-4017(01)00518-0
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发表时间：
2001-12-03
影响因子：
2.6
通讯作者：
Macchioni, G
中科院分区：
文献类型：
--
作者：
Tarantino, C;Rossi, G;Macchioni, G
一只9个月大的阿根廷杜高犬被诊断出患有婴儿利什曼原虫（地中海国家犬利什曼病的病原体）和犬新孢子虫（最近认识到的犬原生动物病原体）。皮肤病变和神经系统体征均存在。皮损的组织学检查显示化脓性弥漫性皮炎伴大量细胞内原虫。血清学检查均为阳性。infantum（1：640）和N.犬（1：800）。皮肤标本的免疫组化双标染色。感染犬巨噬细胞内原虫呈阳性，而N.犬血管内皮细胞和成纤维细胞原虫阳性反应。透射电子显微镜（TEM）证实了这两种原虫的感染。据作者所知，这是第一例同时感染L。infantum和N.一只狗的犬齿利什曼原虫感染或长期类固醇治疗的免疫抑制作用可能是导致N。在这只狗身上。(C)2001 Elsevier Science B.V.保留所有权利。
Leishmania infantum, the agent of canine leishmaniasis in Mediterranean countries, and Neospora caninum, a recently recognized protozoal pathogen in dogs, were diagnosed in a 9-month-old Argentine Dogo dog. Both skin lesions and neurological signs were present. Histopathology of cutaneous lesions revealed a suppurative, diffuse dermatitis with numerous intracellular protozoa. Serology was positive for both L. infantum (1:640) and N. caninum (1:800). Double-label immunohistochemical staining of skin samples with hyperimmune serum from L. infantum-infected dogs was positive for protozoa within macrophages, while the polyclonal antibody specific for N. caninum showed positive reactions for protozoa in endothelial cells and fibroblasts. Transmission electron microscopy (TEM) confirmed the infection with both protozoa. This is, to the authors' knowledge, the first case of simultaneous infection with L. infantum and N. caninum in a dog. It is possible that the immunosuppressive effects of Leishmania infection or long-term steroid therapy may have been a contributing factor to the development of N. caninum in this dog. (C) 2001 Elsevier Science B.V. All rights reserved.