Combined effects of quercetin and curcumin on anti-inflammatory and antimicrobial parameters in vitro
Combined effects of quercetin and curcumin on anti-inflammatory and antimicrobial parameters in vitro
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DOI：
10.1016/j.ejphar.2019.172486
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发表时间：
2019-09-15
影响因子：
5
通讯作者：
Yalcin, A. Suha
中科院分区：
文献类型：
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作者：
Guran, Mumtaz;Sanliturk, Gizem;Yalcin, A. Suha
在这项体外研究中，研究了槲皮素(Q)和姜黄素(C)的联合抗炎作用以及它们对耐甲氧西林金黄色葡萄球菌的联合抗菌活性。观察(I)COX-2表达、(Ii)核因子-kappa-β活化和(Iii)NO水平的抗炎标志物。采用棋盘法检测其抗菌增效作用。我们发现，Q和C合用的“低浓度联合组”(QC)显著下调了COX-2的表达(P<；0.0001)，并抑制了细胞中的核因子-kappa-β的激活(P<；0.0001)，其程度与高浓度Q和C单独作用的程度相似。研究还发现，QC治疗可显著减少NO的产生(P<；0.0001)。与单用QC相比，QC在降低总NO水平方面明显更有效(P<；0.001)。棋盘试验表明，与单独使用最低抑菌浓度相比，Q和C在较低稀释度下对MRSA具有更好的杀灭作用。这些结果表明，与单独使用两种药物相比，低浓度的Q和C联合使用可以产生类似或更好的抗炎效果。此外，它们在抗菌活性方面具有协同作用，与高浓度的Q或C单独使用相比，效果更好。
In this in-vitro study, combinatory anti-inflammatory interactions between Quercetin (Q) and Curcumin (C) along with their combined antimicrobial activity against MRSA were studied. Anti-inflammatory markers of (i) COX-2 expression, (ii) NF kappa beta activation and (iii) NO levels were investigated. Antimicrobial synergy was tested by checkerboard assay. We found that, treatment with the "low-concentration combination group" (QC), where Q and C were combined, resulted in significant downregulation of COX-2 expression (P < 0.0001) and inhibition of NF kappa beta activation in cells (P < 0.0001), to a similar extent to that induced by higher concentrations of Q and C alone. QC treatment was also found to induce a significant reduction in NO production (P < 0.0001). QC was significantly more effective in the reduction of total NO levels when compared to Q alone (P < 0.001). Checkerboard assay indicated that the combination of Q and C provides better killing of MRSA in lower dilutions than standalone Minimum Inhibitory Concentrations. These results suggest that combining low concentrations of Q and C yield similar or better anti-inflammatory effectiveness when compared to treatment with each agent alone. Moreover, they co-operate synergistically in the context of antimicrobial activity, with an increased effectiveness when compared to Q or C alone at high concentrations.