DNA methylation of multiple promoter-associated CpG islands in meningiomas:: relationship with the allelic status at 1p and 22q
DNA methylation of multiple promoter-associated CpG islands in meningiomas:: relationship with the allelic status at 1p and 22q
复制标题
DOI：
10.1007/s00401-004-0911-6
复制
发表时间：
2004-11-01
影响因子：
12.7
通讯作者：
Rey, JA
中科院分区：
文献类型：
--
作者：
Bello, MJ;Amiñoso, C;Rey, JA
本研究的目的是检测脑膜瘤的DNA甲基化谱。因此，我们使用甲基化特异性PCR和测序检测了98例脑膜瘤（68例I级; 27例II级; 3例III级样本）中10个肿瘤相关基因（RB 1、p16（INK 4a）、p73、MGMT、ER、DAPK、TIMP-3、p14（ARF）、THBS 1和Caspase-8）的DNA甲基化状态。最常见的甲基化基因是THBS 1（30%）、TIMP-3（24%）、p16（INK 4a）（17%）、MGMT（16%）、p73（15%）、ER（15%）和p14（ARF）（13%），而其他基因甲基化相对较少（
The purpose of this research was to examine the DNA methylation profile of meningiomas. Accordingly, we examined the DNA methylation status of ten tumor-related genes (RB1, p16(INK4a), p73, MGMT, ER, DAPK, TIMP-3, p14(ARF), THBS1, and Caspase-8) in 98 meningiomas (68 grade I; 27 grade II; and 3 grade III samples) using methylation-specific PCR and sequencing. The most frequently methylated genes were THBS1 (30%), TIMP-3 (24%), p16(INK4a) (17%), MGMT (16%), p73 (15%), ER (15%), and p14(ARF) (13%), whereas methylation was relatively rare in the other genes (