WATER-CONTENT ALTERS VISCOELASTIC BEHAVIOR OF THE NORMAL ADOLESCENT RABBIT MEDIAL COLLATERAL LIGAMENT
WATER-CONTENT ALTERS VISCOELASTIC BEHAVIOR OF THE NORMAL ADOLESCENT RABBIT MEDIAL COLLATERAL LIGAMENT
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DOI：
10.1016/0021-9290(92)90223-n
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发表时间：
1992-08-01
影响因子：
2.4
通讯作者：
BRAY, R
中科院分区：
文献类型：
--
作者：
CHIMICH, D;SHRIVE, N;BRAY, R
测试环境是影响结缔组织力学测试结果的重要因素。本研究的目的是通过改变测试环境来确定韧带水分含量对韧带力学行为的影响。将19只3月龄新西兰大白兔的内侧副韧带(MCL)浸泡在2%、10%、25%蔗糖或0.9%磷酸盐缓冲盐水(PBS)溶液中1h，一侧膝关节在指定的溶液(实验)中循环50次，对侧膝关节(非循环对照)同时在同一水槽中浸泡。训练结束后，分别测定实验韧带和对照韧带的水分含量。22只正常MCL在宰杀后立即测定其水分含量，作为“正常水分”的对照。归一化的峰值循环载荷变化被用作衡量每个MCL的粘弹性行为。结果表明，只有在10%蔗糖溶液中浸泡(但不循环)的韧带的含水率(60.5+/-2.5%)与22个新鲜正常MCL(63.9+/-6.0%)的含水率(63.9+/-6.0%)相似。经PBS(74.0+/-1.3%)或2%蔗糖(69.2+/-2.3%)浸泡的韧带组织含水量明显高于新鲜正常MCL。高水分含量的韧带(如浸泡在PBS或2%蔗糖中)比低水分含量的韧带(如浸泡在25%或10%蔗糖中)表现出更大的循环负荷松弛。不同的流体测试环境会显著改变韧带的水分含量，进而显著影响韧带的粘弹性行为。
Testing environment is an important factor in the outcome of mechanical tests on connective tissue. The purpose of this investigation was to determine the effect of ligament water content on ligament mechanical behaviour by altering the test environment. Water content of medial collateral ligament (MCLs) from 19 three-month-old New Zealand White rabbits was varied in subsets of ligaments pairs by means of immersion in 2, 10 or 25% sucrose or 0.9% phosphate-buffered saline (PBS) solutions for 1 h. One knee joint was cycled 50 times in the designated solution (experimental), while the contralateral knee (uncycled control) was simultaneously soaked in the same tank. Following cycling, the water contents of both test and control ligaments were determined. Water contents of 22 normal MCLs were determined immediately post-sacrifice and served as 'normal water content' controls. Normalized peak cyclic load changes were used as a measure of the viscoelastic behaviour of each MCL. Results demonstrated that only ligaments soaked (but not cycled) in a 10% sucrose solution had water contents (60.5+/-2.5%) which were statistically similar to the 22 fresh normal MCLs (63.9+/-6.0%). Ligaments soaked in PBS (74.0+/-1.3 %) or 2% sucrose (69.2+/-2.3%) had significantly higher water contents compared to fresh normal MCLs. Ligaments with higher water contents (e.g. soaked in PBS or 2% sucrose) demonstrated greater cyclic load relaxation compared to ligaments with lower contents (e.g. soaked in 25 or 10% sucrose). Different fluid test environments can significantly alter ligament water content and, in turn, significantly affect ligament viscoelastic behaviour.