Generation and amplification of temperature-sensitive mutants during serial undiluted passages of vesicular stomatitis virus.
Generation and amplification of temperature-sensitive mutants during serial undiluted passages of vesicular stomatitis virus.
复制标题
水泡性口炎病毒连续未稀释传代过程中温度敏感突变体的产生和扩增。
DOI：
10.1016/0042-6822(81)90529-8
复制
发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
Frielle,DW
中科院分区：
文献类型：
--
作者：
Youngner,JS;Jones,EV;Kelly,M;Frielle,DW
Serial undiluted passages of wild-type (wt) vesicular stomatitis virus (VSV) produce a striking increase in the frequency of temperature-sensitive (ts) mutants in the virus population. The host cell and, in some instances, pretreatment with actinomycin D have a marked influence on this phenomenon. The increase in ts mutants is not correlated with the production of defective-interfering (DI) particles in the same virus population. BHK-21 cells, which are efficient producers of DI particles (with or without actinomycin D pretreatment), fail to produce increased frequencies of ts mutants except when pretreated with actinomycin D. In contrast, L cells which are poor producers of DI particles, are very efficient producers of ts mutants when the serial undiluted passages are carried out with or without actinomycin D pretreatment. The MDBK line of bovine kidney cells fails to produce DI particles or ts mutants through 10 serial undiluted passages made in cells pretreated or not with actinomycin D. These results suggest that separate host factors are involved in the production of deletion mutants (DI particles) and base change mutants (ts mutants). It is suggested that there may be a host cell factor involved in the fidelity of RNA transcription. Less stringent control of this process in certain hosts (L cells) or the repression of a “fidelity factor” by actinomycin D (BHK-21 cells) may permit errors to be made when a large load of RNA in the form of viral genomes is being transcribed. It is proposed that these errors cause base changes in the virion genomes which are replicated and are expressed phenotypically as temperature-sensitive mutants.
