Near-Infrared Light-Excited Upconverting Persistent Nanophosphors in Vivo for Imaging-Guided Cell Therapy
Near-Infrared Light-Excited Upconverting Persistent Nanophosphors in Vivo for Imaging-Guided Cell Therapy
复制标题
近红外光激发的体内上转换持久性纳米磷光体用于成像引导细胞治疗
DOI：
10.1021/acsami.8b05706
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发表时间：
2018-06-13
影响因子：
9.5
通讯作者：
Chang, Jin
中科院分区：
文献类型：
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作者：
Zheng, Bin;Bai, Yang;Chang, Jin
光学成像在生物应用中需要更灵敏的工具。持久发光的纳米荧光粉能够实现高度灵敏的体内光学检测，并且几乎完全避免组织自发荧光。然而，实际的持久发光纳米荧光粉在全身操作之前需要离体激活，这严重限制了体内长期成像的使用。因此，我们介绍了一种新一代的光学纳米荧光粉，基于（Zn 2SiO 4：Mn）：Y3+，Yb 3+，Tm 3+上转换持久发光纳米荧光粉;这些纳米荧光粉可以在体内通过活组织被高度穿透的近红外光激发。我们可以在体外内吞这些纳米磷光体后在体内追踪标记的肿瘤治疗性巨噬细胞，并通过简单的整个动物光学检测来跟踪巨噬细胞的生物分布。这些纳米荧光粉将为细胞治疗研究和体内诊断的各种应用开辟新的潜力。
Optical imaging for biological applications is in need of more sensitive tool. Persistent luminescent nanophosphors enable highly sensitive in vivo optical detection and almost completely avoid tissue autofluorescence. Nevertheless, the actual persistent luminescent nanophosphors necessitate ex vivo activation before systemic operation, which severely restricted the use of long-term imaging in vivo. Hence, we introduced a novel generation of optical nanophosphors, based on (Zn2SiO4:Mn):Y3+, Yb3+, Tm3+ upconverting persistent luminescent nanophosphors; these nanophosphors can be excited in vivo through living tissues by highly penetrating near-infrared light. We can trace labeled tumor therapeutic macrophages in vivo after endocytosing these nanophosphors in vitro and follow macrophages biodistribution by a simple whole animal optical detection. These nanophosphors will open novel potentials for cell therapy research and for a variety of applications in diagnosis in vivo.