AXON-REGULATED EXPRESSION OF A SCHWANN-CELL TRANSCRIPT THAT IS HOMOLOGOUS TO A GROWTH ARREST-SPECIFIC GENE
AXON-REGULATED EXPRESSION OF A SCHWANN-CELL TRANSCRIPT THAT IS HOMOLOGOUS TO A GROWTH ARREST-SPECIFIC GENE
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DOI：
10.1002/j.1460-2075.1991.tb04933.x
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发表时间：
1991-12-01
期刊：
影响因子：
11.4
通讯作者：
MULLER, HW
中科院分区：
文献类型：
--
作者：
SPREYER, P;KUHN, G;MULLER, HW
我们已经分离出1.8 kb的cDNA （pCD25）克隆，该克隆编码在神经再生过程中差异表达的转录物。核苷酸序列比较表明，该基因与最近发现的小鼠“生长抑制特异性”基因gas3同源性为89.6%。CD25转录物的开放阅读框预测一个17 kDa的蛋白具有四个假定的跨膜区域。CD25 mRNA在坐骨神经中的稳态水平远高于其他组织，并且在坐骨神经中的表达仅限于雪旺细胞。神经损伤后，远端神经段的转录物水平迅速下降，但在神经再生后恢复。相比之下，在永久切断的神经远端残端，mRNA水平仍然很低。大量的mRNA只有在这些中断的神经残端吻合时才能被重新诱导。mRNA的再诱导是随着再生轴突通过远端神经段的延伸而发生的。我们的数据表明，轴突调节了雪旺细胞中CD25 mRNA的表达，表明CD25蛋白在雪旺细胞生长和分化过程中起作用。
We have isolated a 1.8 kb cDNA (pCD25) clone that encodes a transcript that is differentially expressed during nerve regeneration. Nucleotide sequence comparison indicates 89.6% homology with the recently identified murine 'growth arrest-specific' gene gas3. The open reading frame of the CD25 transcript predicts a 17 kDa protein with four putative transmembrane regions. Steady-state levels of the CD25 mRNA are verv much higher in sciatic nerve than in other tissues, and expression in sciatic nerve is confined to Schwann cells. Following nerve injury, the transcript levels rapidly declined in nerve segments distal to the site of lesion, but recovered upon nerve regeneration. In contrast, in distal stumps of permanently transected nerves, the mRNA level remained very low. Substantial amounts of the mRNA could be reinduced only upon anastomosis of these interrupted nerve stumps. Re-induction of the mRNA followed the elongation of regenerating axons through the distal nerve segment. Our data indicate that axons regulate expression of the CD25 mRNA in Schwann cells, and suggest that the CD25 protein functions during Schwann cell growth and differentiation.