Patterning mammalian cells using elastomeric membranes
Patterning mammalian cells using elastomeric membranes
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DOI：
10.1021/la000382m
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发表时间：
2000-10-03
期刊：
影响因子：
3.9
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Ostuni, E;Kane, R;Whitesides, GM
我们描述的图案的蛋白质和细胞的表面上的细菌培养皿，玻璃，和聚（二甲基硅氧烷）（PDMS）与使用弹性剥离膜-独立的聚合物膜，具有圆形或方形孔的直径，侧面，和高度大于或等于50 μ m。细胞在由膜的孔提供的物理约束内形成图案;这些约束可以被释放以允许细胞扩散到表面的其余部分上或通过控制表面的性质而保持图案。需要仔细控制基底表面的性质，以使细胞粘附于基底而不是膜，并避免在去除膜时对细胞的损害。这种基于膜的图案化策略（缩写为MEMPAT）提供了一种比现有方法更方便的方法来在表面上图案化细胞和研究细胞扩散。
We describe the patterning of proteins and cells onto the surfaces of bacteriological Petri dishes, glass, and poly(dimethylsiloxane) (PDMS) with the use of elastomeric lift-off membranes-free-standing polymer films that have circular or square holes with diameters, sides, and height greater than or equal to 50 mu m. Cells are patterned within the physical constraints provided by the holes of the membranes; these constraints can be released to allow the cells to spread onto the rest of the surface or to remain in the pattern by controlling the properties of the surfaces. Careful control of the properties of the surfaces of the substrates are required to cause the cells to adhere to the substrate and not to the membrane, and to avoid damage to the cells on removing the membrane. This strategy of membrane-based patterning-given the acronym MEMPAT for brevity-offers a more convenient way for patterning cells on surfaces and for studying cell spreading than existing methods.