Drosophila growth-blocking peptide-like factor mediates acute immune reactions during infectious and non-infectious stress.
Drosophila growth-blocking peptide-like factor mediates acute immune reactions during infectious and non-infectious stress.
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DOI：
10.1038/srep00210
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发表时间：
2012
影响因子：
4.6
通讯作者：
Hayakawa Y
中科院分区：
文献类型：
--
作者：
Tsuzuki S;Ochiai M;Matsumoto H;Kurata S;Ohnishi A;Hayakawa Y
抗菌肽（Antimicrobial Peptides，AMPs）是机体主要的天然免疫效应物，具有保护机体免受微生物侵害的作用. AMP也在非感染性应激下被诱导;然而，非感染性应激诱导的AMP表达的信号通路尚不清楚。我们证明，生长阻断肽（GBP）是一种有效的细胞因子，调节昆虫应激诱导的AMP表达。GBP在果蝇中的过表达增加了AMP的表达。GBP诱导的AMP表达不需要Toll和免疫缺陷（Imd）通路相关的基因，但imd和篮子是必不可少的，这表明GBP信号在果蝇中不使用传统的Toll或Imd通路，但使用JNK通路后，与接头蛋白Imd。非感染性的物理或环境压力的AMP表达的增强是明显的控制，但不是在GBP敲低幼虫。这些结果表明，果蝇GBP信号通路介导的急性先天免疫反应在各种压力下，无论他们是感染性或非感染性。
Antimicrobial peptides (AMPs), major innate immune effectors, are induced to protect hosts against invading microorganisms. AMPs are also induced under non-infectious stress; however, the signaling pathways of non-infectious stress-induced AMP expression are yet unclear. We demonstrated that growth-blocking peptide (GBP) is a potent cytokine that regulates stressor-induced AMP expression in insects. GBP overexpression in Drosophila elevated expression of AMPs. GBP-induced AMP expression did not require Toll and immune deficiency (Imd) pathway-related genes, but imd and basket were essential, indicating that GBP signaling in Drosophila did not use the orthodox Toll or Imd pathway but used the JNK pathway after association with the adaptor protein Imd. The enhancement of AMP expression by non-infectious physical or environmental stressors was apparent in controls but not in GBP-knockdown larvae. These results indicate that the Drosophila GBP signaling pathway mediates acute innate immune reactions under various stresses, regardless of whether they are infectious or non-infectious.