NUCLEOSOME STRUCTURE STUDIED WITH PURIFIED ANTIBODIES TO HISTONES-H2B, HISTONES-H3 AND HISTONES-H4
NUCLEOSOME STRUCTURE STUDIED WITH PURIFIED ANTIBODIES TO HISTONES-H2B, HISTONES-H3 AND HISTONES-H4
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DOI：
10.1016/0014-5793(78)81248-4
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发表时间：
1978-01-01
期刊：
影响因子：
3.5
通讯作者：
VANREGENMORTEL, MHV
中科院分区：
文献类型：
--
作者：
ABSOLOM, D;VANREGENMORTEL, MHV
染色质的基本结构似乎由一串称为核小体的重复单元组成 [1]，其中包含两个组蛋白 H2A、H2B、H3 和 H4 分子以及大约 200 个碱基对的 DNA [2--51。 Nudeosornes 可以通过用微球菌色调短暂消化染色质来获得[容易，平均直径为 1.?~，在等速密度梯度中沉积在 11 S：~ 6]。针对 dL'Ēerent 组蛋白的特异性抗血清的可用性提高了通过免疫化学方法研究核体形貌的可能性 [7]。如果可以定位组蛋白的抗原决定簇区域[8--10]并制备相应的抗体片段，则可以确定核小体内特定组蛋白的精确方向。我们现在报告了小牛胸腺组蛋白 H2B 中一种抗原决定因素的位置，并举例说明了使用纯化的 FI2t~、H3 和 H4 抗体来阐明核小体的表面结构_
The basic strtxcture of chromatin appears to consist of a string of repeating units called nucleosomes [1] which contain two molecules each of the histones H2A, H2B, H3 and H4 and about 200 base pair~ of DNA [2--51. Nudeosornes which can be obtained by brief digestion of chromatin with mic~ ococcal hue [ease, have an average diameter of 1.?~ and sediment at 11 S in isokinetic density gradiet.:~ 6]. The availability of specific antisera to the dL'Ēerent histones raises the possibility of studying the topography of the nudeosome by immunochemical methods [7]. If the antigenic determinant regions of histones could be located [8--10] and the corresponding antibody fractions prepared, it may become possible to determine the precise orientation of particular histones within the nucleosome. We now report on the location of one antigenic determhwmt in histone H2B of calf thymus, and illustrate the use of purified FI2t~, H3 and H4 antibodies for elucidating the surface structure of nucleosomes_