An enhancer sequence from bovine papilloma virus DNA consists of two essential regions.
An enhancer sequence from bovine papilloma virus DNA consists of two essential regions.
复制标题
牛乳头状瘤病毒 DNA 的增强子序列由两个必需区域组成。
DOI：
10.1093/nar/12.6.2901
复制
发表时间：
1984
影响因子：
14.9
通讯作者：
Botchan,MR
中科院分区：
文献类型：
--
作者：
Weiher,H;Botchan,MR
基于20个突变体衍生物的构建和功能分析，对牛乳头瘤病毒DNA增强子序列进行了全面的结构分析。在CV-1组织培养细胞中获得的结果表明，该增强子是一个小的遗传元件-长度仅为40 bp-包含两个必需区域。这两个区域显示出彼此同源性以及与来自其他病毒基因组的DNA片段的同源性，所述其他病毒基因组的DNA片段也在所使用的测定中充当增强子。然而，在CV-1细胞中具有增强子活性的序列中存在一定的自由度，甚至在关键区域内。结果进行了讨论的模型，增强子是组织特异性转录因子的结合位点。Z-DNA的形成可能参与了增强过程。然而，在BPV增强子内的交替嘌呤和嘧啶的8个碱基对延伸中的单碱基对转换（其保存了这种模式）破坏了增强子功能。
A comprehensive structural analysis of an enhancer sequence from bovine papilloma virus DNA is presented based on the construction and functional analysis of 20 mutant derivatives. The results, obtained in CV-1 tissue culture cells, show that this enhancer is a small genetic element — only 40 bp in length — that contains two essential regions. The two regions exhibit homology to each other and to DNA fragments from other viral genomes that also act as enhancers in the assay used. However, there is a certain latitude in the sequences that have enhancer activity in CV-1 cells, even within the critical regions. The results are discussed with respect to the model that enhancers are binding sites for tissue specific transcription factors. The formation of Z-DNA might be involved in the enhancement process. However, single base pair transitions in an 8 base pair stretch of alternating purines and pyrimidines within the BPV enhancer which conserve this pattern destroy enhancer function.