Simulated microgravity culture system for a 3-D carcinoma tissue model
Simulated microgravity culture system for a 3-D carcinoma tissue model
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DOI：
10.2144/02335rr02
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发表时间：
2002-11-01
期刊：
影响因子：
2.7
通讯作者：
Katano, M
中科院分区：
文献类型：
--
作者：
Nakamura, K;Kuga, H;Katano, M
需要体外器官培养模型来了解由癌细胞、基质细胞和细胞外基质组成的癌组织的复杂性。我们使用具有四个一次性容器（RCCS™-4D）的旋转细胞培养系统开发了一种新的癌组织体外模型，该系统提供模拟微重力条件。将含有人胰腺癌NOR-P1细胞和成纤维细胞的固体胶原凝胶或切碎的人胰腺癌组织在模拟微重力条件或静态1g条件下培养7天。与静态 1g 条件下的培养物相比，模拟微重力条件下的 NOR-P1 培养物显示出更多数量的有丝分裂细胞、循环细胞（Ki-67 阳性）、核因子 kappaB 激活细胞，以及更少数量的凋亡细胞。此外，在模拟微重力条件下培养的人胰腺癌标本比静态培养条件更好地保持了原始癌组织的异质组成和细胞活性（由循环细胞比率和有丝分裂指数决定）。这种具有四个处置容器的新型 3D 旋转细胞培养系统可能有助于复杂胰腺癌组织的体外研究。
An in vitro organotypic culture model is needed to understand the complexities of carcinoma tissue consisting of carcinoma cells, stromal cells, and extracellular matrices. We developed a new in vitro model of carcinoma tissue using a rotary cell culture system with four disposable vessels (RCCS(TM)-4D) that provides a simulated microgravity condition. Solid collagen gels containing human pancreatic carcinoma NOR-P1 cells and fibroblasts or minced human pancreatic carcinoma tissue were cultured under a simulated microgravity condition or a static 1g condition for seven days. NOR-P1 cultures subjected to the simulated microgravity condition showed greater numbers of mitotic, cycling (Ki-67-positive), nuclear factor-kappaB-activating cells, and a lower number of apoptotic cells than were shown by cultures subjected to the static 1g condition. In addition, human pancreatic carcinoma specimens cultured under the simulated microgravity condition maintained the heterogeneous composition and cellular activity (determined by the cycling cell ratio and mitotic index) of the original carcinoma tissue better than static culture conditions. This new 3-D rotary cell culture system with four disposal vessels may be useful for in vitro studies of complex pancreatic carcinoma tissue.