Detection of the photoactive protochlorophyllide‐protein complex in the light during the greening of barley
Detection of the photoactive protochlorophyllide‐protein complex in the light during the greening of barley
复制标题
大麦变绿过程中光活性原叶绿素-蛋白质复合物的检测
DOI：
10.1016/0014-5793(92)80742-y
复制
发表时间：
1992
期刊：
影响因子：
3.5
通讯作者：
K. Strzałka
中科院分区：
文献类型：
--
作者：
F. Franck;K. Strzałka
在绿色大麦叶中检测到在648和653 nm处具有吸光度和荧光最大值的光活性原叶绿素-蛋白质复合物，而没有任何再变暗。在两种不同的光强度下，光活性原叶绿素的吸光度和荧光的振幅随绿化时间的变化表明，在第一个小时内，叶绿素合成的速率和复合物的量之间存在密切的关系。在绿化过程中由光还原产生的叶绿素在684 nm处具有最大吸光度，其在几秒钟内向较短波长移动，表明色素从酶中快速释放。我们的结论是，叶绿素积累的过程中，通过不断再生和光转化的光活性复合物。
A photoactive protochloraphyllide‐protein complex with absorbance and flourescence maxima at 648 and 653 nm was detected in greening barley leaves without any re‐darkening. The variations of the amplitudes of the absorbance and the fluorescence of the photoactive protochlorophyllide with greening time at two different light intensities indicate a close relationship between the rate of chlorophyll synthesis and the amount of the complex during the first hours. The chlorophyllide resulting from photoreduction during greening has an absorbance maximum at 684 nm, which shifts towards a shorter wavelength within a few seconds, indicating rapid liberation of the pigment from the enzyme. We conclude that chlorophyll accumulation proceeds through continuous regeneration and phototransformation of the photoactive complex.