Role of lipopolysaccharide in the induction of type I interferon-dependent cross-priming and IL-10 production in mice by meningococcal outer membrane vesicles
Role of lipopolysaccharide in the induction of type I interferon-dependent cross-priming and IL-10 production in mice by meningococcal outer membrane vesicles
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DOI：
10.1016/j.vaccine.2009.01.109
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发表时间：
2009-03-18
期刊：
影响因子：
5.5
通讯作者：
Wong, Simon Y. C.
中科院分区：
文献类型：
--
作者：
Durand, Vanessa;MacKenzie, Joanne;Wong, Simon Y. C.
我们研究了脂多糖（LPS）对脑膜炎球菌B病候选疫苗天然外膜囊泡（NOMV）佐剂特性的贡献。NOMV通过Toll样受体（TLR）2和4诱导小鼠骨髓来源的树突状细胞的成熟和细胞因子产生，Toll样受体（TLR）2和4主要依赖于信号转导衔接子MyD 88。NOMV还能够诱导来自LPS低应答小鼠的脾细胞中的B细胞增殖。然而，NOMV在体内诱导IL-10和I型干扰素依赖性、抗原特异性和分泌IFN γ的CD 8（+）细胞毒性T淋巴细胞应答需要LIPS。LPS在诱导IL-10和功能性交叉引发中的重要性对基于NOMV的疫苗和佐剂的开发具有意义。(C)2009爱思唯尔有限公司保留所有权利。
We investigated the contribution of lipopolysaccharide (LPS) to adjuvant properties of native outer membrane vesicles (NOMV), a vaccine candidate for meningococcal B disease. NOMV induce the maturation of and cytokine production by murine bone marrow-derived dendritic cells through both toll-like receptors (TLR) 2 and 4 which are mostly dependent on the signalling adaptor MyD88. NOMV are also able to induce B cell proliferation in splenocytes from LPS-hyporesponsive mice. However, induction of IL-10 and type I interferon-dependent, antigen-specific and IFN gamma-secreting CD8(+) cytotoxic T lymphocyte responses in vivo by NOMV requires LIPS. The importance of LPS in the induction of IL-10 and functional cross-priming has implications for NOMV-based vaccine and adjuvant development. (C) 2009 Elsevier Ltd. All rights reserved.