CDNA CLONING OF A MOUSE MAMMARY EPITHELIAL-CELL SURFACE PROTEIN REVEALS THE EXISTENCE OF EPIDERMAL GROWTH FACTOR-LIKE DOMAINS LINKED TO FACTOR-VIII-LIKE SEQUENCES
CDNA CLONING OF A MOUSE MAMMARY EPITHELIAL-CELL SURFACE PROTEIN REVEALS THE EXISTENCE OF EPIDERMAL GROWTH FACTOR-LIKE DOMAINS LINKED TO FACTOR-VIII-LIKE SEQUENCES
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小鼠乳腺上皮细胞表面蛋白的 cdna 克隆揭示了表皮生长因子样结构域与因子-viii 样序列的联系
DOI：
10.1073/pnas.87.21.8417
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发表时间：
1990-11-01
影响因子：
11.1
通讯作者：
PARRY, G
中科院分区：
文献类型：
--
作者：
STUBBS, JD;LEKUTIS, C;PARRY, G
从小鼠乳腺Lambda.g11文库中分离到一个编码乳腺上皮细胞表面蛋白的2.1kb碱基的cDNA.序列分析表明，该基因有一个1389个碱基对的开放阅读框，定义了一个相对分子质量为51.5 kDa的蛋白质。对预测序列的结构分析确定了该蛋白的两个可能的功能结构域：(I)与果蝇Notch-1蛋白的表皮生长因子样结构域相似的N-末端富含半胱氨酸的区域；(Ii)该序列的一大段与人凝血因子VIII和V的C-末端结构域具有54.5%的同源性。这些结构上的相似性被用来预测该蛋白可能的功能及其与细胞表面的相互作用方式。在非妊娠动物的乳腺组织中可以检测到mRNA的表达，但在哺乳腺中的表达最高。在培养细胞中，信使核糖核酸水平也与细胞分化程度相关。
A 2.1-kilobase cDNA coding for a surface protein of mammary epithelial cells has been isolated from a mouse mammary gland .lambda.g11 cDNA library. Sequence analysis of this cDNA reveals an open reading frame of 1389 base pairs that defines a protein with a molecular mass of 51.5 kDa. Structural analysis of the predicted sequence identifies two putative functional domains of the protein: (i) an N-terminal cysteine-rich region that is similar to epidermal growth factor-like domains of Drosophila Notch-1 protein and (ii) a large segment of the sequence that exhibited 54.5% identity with C-terminal domains of human coagulation factors VIII and V. These similarities in structure are used to predict the possible functions of the protein and its means of interaction with the cell surface. mRNA expression was detectable in mammary tissue from nonpregnant animals but was maximal in the lactating gland. In cultured cells, mRNA levels also correlated with the degree of cellular differentiation.