MicroRNA-based molecular markers: a novel PCR-based genotyping technique in Brassica species
MicroRNA-based molecular markers: a novel PCR-based genotyping technique in Brassica species
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基于 MicroRNA 的分子标记：一种新型的基于 PCR 的芸苔属基因分型技术
DOI：
10.1111/pbr.12069
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发表时间：
2013-08-01
期刊：
影响因子：
2
通讯作者：
An, Zeshan
中科院分区：
文献类型：
--
作者：
Fu, Donghui;Ma, Bi;An, Zeshan
基于DNA的分子标记在基因定位、遗传多样性分析、种质评价和分子标记辅助选择等方面发挥着重要作用。利用传统的分子标记体系很难实现多态丰富、稳定性好、生产容易、效率高等理想的标记特性的结合。MicroRNAs是一种广泛存在于多种生物基因组中的内源性非编码RNA。MiRNA和前miRNA序列的高度保守性为开发新型分子标记类型提供了机会。我们从Brassica miRBase数据库中下载了82个miRNA序列，设计了46个基于miRNA的单引物，可以随机组合产生引物对。利用来自6个油菜属16个品种的DNA，验证了其中一部分引物的可转移性和多态性。34对引物的多态信息含量平均值为0.43，多态率为28.1%。在物种间证实了良好的可转移性。这些结果表明，基于miRNA的标记提供了一种新的基因分型技术，具有成本低、效率高、稳定性好和可移植性好的特点。
DNA-based molecular markers play a significant role in gene mapping, genetic diversity analysis, germplasm evaluation and molecular marker-assisted selection. A combination of desirable marker characteristics such as abundant polymorphism, good stability, ease of production and high efficiency is difficult to achieve when utilizing traditional molecular marker systems. MicroRNAs are a type of endogenous non-coding RNAs prevalent in the genomes of many organisms. The high conservation of miRNA and pre-miRNA sequences provides an opportunity to develop a novel molecular marker type. We downloaded 82 miRNA sequences from the Brassica miRBase database and designed 46 single miRNA-based primers which could be randomly combined to generate primer pairs. A proportion of these primer pairs were validated for transferability and polymorphism using DNA from 16 varieties of six Brassica species. The percentage of polymorphic primer pairs were 28.1%, and the average polymorphism information content (PIC) value for the 34 primer pairs was 0.43. Good transferability was verified across species. These results indicate that miRNA-based markers provide a novel genotyping technique with low costs, high efficiency, stability and good transferability.