Transfer RNA Derived Small RNAs Targeting Defense Responsive Genes Are Induced during Phytophthora capsici Infection in Black Pepper (Piper nigrum L.).
Transfer RNA Derived Small RNAs Targeting Defense Responsive Genes Are Induced during Phytophthora capsici Infection in Black Pepper (Piper nigrum L.).
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DOI：
10.3389/fpls.2016.00767
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发表时间：
2016
影响因子：
5.6
通讯作者：
Soniya EV
中科院分区：
文献类型：
--
作者：
Asha S;Soniya EV
在本研究中，从转移RNA中衍生的小型RNA被指定为各种压力反应，我们报告了源自黑胡椒植物的tRNA，响应于快速湿润的病原体，phytophthora capsici pecpers pecpers peppers peppers peppers。吞吐量系统对小RNAome（SRNAOME）的分析表明，在感染的叶片和根中，5'TRF在SRNAOME中的5'TRF和防御响应基因的潜在靶标表示其在5'Alacgc Traf the nortimed Cleagage the nortimentiment of traf nounteriment traf nounteriment traf nounteriment traf nounteriment MR的含量中，因此srnaome的抽象表明其调节作用。蛋白质（NPR1），下调在病原体感染期间。
Small RNAs derived from transfer RNAs were recently assigned as potential gene regulatory candidates for various stress responses in eukaryotes. In this study, we report on the cloning and identification of tRNA derived small RNAs from black pepper plants in response to the infection of the quick wilt pathogen, Phytophthora capsici. 5′tRFs cloned from black pepper were validated as highly expressed during P. capsici infection. A high-throughput systematic analysis of the small RNAome (sRNAome) revealed the predominance of 5′tRFs in the infected leaf and root. The abundance of 5′tRFs in the sRNAome and the defense responsive genes as their potential targets indicated their regulatory role during stress response in black pepper. The 5′AlaCGC tRF mediated cleavage was experimentally mapped at the tRF binding sites on the mRNA targets of Non-expresser of pathogenesis related protein (NPR1), which was down-regulated during pathogen infection. Comparative sRNAome further demonstrated sequence conservation of 5′Ala tRFs across the angiosperm plant groups, and many important genes in the defense response were identified in silico as their potential targets. Our findings uncovered the diversity, differential expression and stress responsive functional role of tRNA-derived small RNAs during Phytophthora infection in black pepper.