Tissue distribution of capilliposide B, capilliposide C and their bioactive metabolite in mice using liquid -tandem mass spectrometry
Tissue distribution of capilliposide B, capilliposide C and their bioactive metabolite in mice using liquid -tandem mass spectrometry
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DOI：
10.1002/bmc.3895
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发表时间：
2017-06-01
影响因子：
1.8
通讯作者：
Jiang, Hongliang
中科院分区：
文献类型：
--
作者：
Cheng, Zhongzhe;Zhou, Xing;Jiang, Hongliang
细茎过路黄（报春花科）是我国民间药用植物，在体内和体外均表现出明显的抗肿瘤活性。毛蕊花甙B（LC-B B）和毛蕊花甙C（LC-C）是该植物中的主要活性成分。为了探索它们的组织分布，开发并验证了用于定量小鼠组织中LC-B、LC-C及其生物活性代谢物毛蕊甙A（LC-A）的可靠生物分析方法。在这项研究中，三个化合物的组织分布曲线进行了检查后，静脉注射纯LC-B和口服给药的总皂苷的L。毛蕊花提取物（LCE）处理10天。在10.0- 5000 ng/mL的曲线范围内对各种组织匀浆中的所有三种分析物进行了方法验证。QC样品的日内和日间精密度的相对标准偏差为
Lysimachia capillipes Hemsl (Primulaceae), a folk medicinal plant in China, showed significant anti-tumor activities in vivo and in vitro. Capilliposide B (LC-B) and capilliposide C (LC-C) are the main bioactive components in this plant. To explore their tissue distribution, a reliable bioanalytical method for the quantification of LC-B, LC-C and their bioactive metabolite, capilliposide A (LC-A), in mouse tissues was developed and validated. In this study, the tissue distribution profiles of the three compounds were examined after intravenous administration of pure LC-B and oral administration of total saponins of L. capillipes Hemsl extract (LCE) for 10days. Method validation was conducted over the curve range 10.0-5000ng/mL for all three analytes in various tissue homogenates. The relative standard deviation of intra-day and inter-day precision of the QC samples was