Occurrence and expression of gene transfer agent genes in marine bacterioplankton
Occurrence and expression of gene transfer agent genes in marine bacterioplankton
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DOI：
10.1128/aem.02129-07
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发表时间：
2008-05-01
影响因子：
4.4
通讯作者：
Moran, Mary Ann
中科院分区：
文献类型：
--
作者：
Biers, Erin J.;Wang, Kui;Moran, Mary Ann
在海洋玫瑰杆菌分支的三个培养成员的基因组序列中发现了与转导类基因转移剂(GTA)同源的基因。对这种宿主控制的病毒样基因转移系统的更广泛的同源搜索发现，在培养的甲蛋白细菌中，特别是在海洋浮游细菌代表中，可能存在GTA系统。实验证明，玫瑰色杆菌分支成员硅杆菌DSS-3的GTA基因表达和GTA颗粒(类似于50~70 nm)的胞外释放，以及种内基因转移。然而，在海洋元基因组序列数据中，GTA同源基因出人意料地罕见，而且这种横向基因转移机制在海洋浮游细菌群落中的作用尚不清楚。
Genes with homology to the transduction-like gene transfer agent (GTA) were observed in genome sequences of three cultured members of the marine Roseobacter clade. A broader search for homologs for this host-controlled virus-like gene transfer system identified likely GTA systems in cultured Alphaproteobacteria, and particularly in marine bacterioplankton representatives. Expression of GTA genes and extracellular release of GTA particles (similar to 50 to 70 nm) was demonstrated experimentally for the Roseobacter clade member Silicibacter pomeroyi DSS-3, and intraspecific gene transfer was documented. GTA homologs are surprisingly infrequent in marine metagenomic sequence data, however, and the role of this lateral gene transfer mechanism in ocean bacterioplankton communities remains unclear.