Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12.
Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12.
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DOI：
10.1111/j.1574-6968.1996.tb08368.x
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发表时间：
1996-07
影响因子：
2.1
通讯作者：
Y. Yang;G. Zhao;M. Winkler
中科院分区：
文献类型：
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作者：
Y. Yang;G. Zhao;M. Winkler
我们分离出一个miniTn 10（CMR）插入突变体缺乏吡哆醇（PN）激酶和克隆的结构基因，指定pdxK，通过互补。P1转导和PCR定位及DNA序列分析表明，pdxK与crr糖转运基因相邻（53.95 min）。pdxK：：miniTn 10突变体的生长特性支持以下假设，即在体外也磷酸化吡哆醛（PL）和吡哆胺（PM）的PN激酶仅在B6-维生素补救途径中起作用，而E. coli中含有另外的PL激酶。PdxK的氨基酸序列具有PfkB超家族碳水化合物激酶的特征基序，该超家族包括磷酸果糖激酶和核糖激酶，并且表明鼠伤寒沙门氏菌、流感嗜血杆菌和酿酒酵母的三个未鉴定的ORF对应于PN/PL/PM激酶。
We isolated a miniTn10(Cmr) insertion mutant lacking pyridoxine (PN) kinase and cloned the structural gene, designated pdxK, by complementation. P1 transduction and PCR mapping and DNA sequence analysis showed that pdxK was adjacent to the crr sugar transport gene (53.95 min). Growth properties of pdxK::miniTn10 mutants supported the hypotheses that PN kinase, which also phosphorylates pyridoxal (PL) and pyridoxamine (PM) in vitro, functions solely in the B6-vitamer salvage pathway and that E. coli contains an additional PL kinase. The amino acid sequence of PdxK has signature motifs of the PfkB superfamily of carbohydrate kinases, which includes phosphofructokinases and ribokinases, and suggests that three unidentified ORFs of Salmonella typhimurium, Haemophilus influenzae, and Saccharomyces cerevisiae correspond to PN/PL/PM kinases.