Vitamins C and E: acute interactive effects on biomarkers of antioxidant defence and oxidative stress
Vitamins C and E: acute interactive effects on biomarkers of antioxidant defence and oxidative stress
复制标题
DOI：
10.1016/j.mrfmmm.2004.03.006
复制
发表时间：
2004-07-13
影响因子：
2.3
通讯作者：
Strain, JJ
中科院分区：
文献类型：
--
作者：
Choi, SW;Benzie, IFF;Strain, JJ
氧化应激与许多疾病的病因有关；然而，大多数抗氧化微量营养素的补充试验并没有显示出预期的有益效果。这项随机、双盲、安慰剂对照研究评估了单剂量维生素C（500毫克）和维生素E（400国际单位）单独和联合使用对12名健康自愿志愿者血浆抗氧化状态、脂质过氧化和淋巴细胞DNA损伤的生物标志物的急性影响（摄入后90、180分钟和24小时[禁食]）。血浆抗坏血酸在摄入维生素C后2 h内显著升高（P < 0.01）， α -生育酚在摄入维生素E后24 h显著升高（P < 0.01）。维生素C（或维生素E）单独或联合服用对α -生育酚的反应模式无显著差异，表明同时摄入维生素C或维生素E不会增强对其中一种维生素的反应。在整个治疗过程中，组内血浆FRAP（尽管在摄入维生素C后的90和180分钟出现了增加（P < 0.05））或MDA均未见显著变化，并且在摄入维生素C和/或E后的任何时间点均未见DNA损伤或DNA保护增加的证据。总之，单剂量维生素C和/或E的急性效应的第一次对照研究的数据显示，没有证据表明对DNA损伤、DNA抗氧化性或脂质过氧化有保护或有害作用。没有证据表明维生素C和维生素E之间存在协同或协同相互作用，但需要进一步研究以确定交错补充周期中可能的相互作用，并且对氧化应激增加或边缘抗氧化状态的受试者进行研究将是有用的。研究这些维生素与全食物一起摄入或在全食物中摄入的效果，以确定它们是否在超过预防缺乏症所需的最低剂量时具有直接保护作用，是否需要与其他食物成分组合以获得有效保护，或者维生素C和E是否在很大程度上是“健康”饮食的替代生物标志物，但不是关键的保护剂，也将是一项有趣的研究。(C) 2004 Elsevier B.V.版权所有
Oxidative stress is implicated in the aetiology of many diseases; however, most supplementation trials with antioxidant micronutrients have not shown expected beneficial effects. This randomized, double-blinded, placebo-controlled study evaluated acute effects (at 90, 180 min and 24 h [fasting] post-ingestion) of single doses of Vitamins C (500 mg) and E (400 IU), alone and in combination, on biomarkers of plasma antioxidant status, lipid peroxidation and lymphocyte DNA damage in 12 healthy, consenting volunteers. Plasma ascorbic acid increased significantly (P < 0.01) within 2 h of ingestion of Vitamin C, and alpha-tocopherol was significantly (P < 0.0 1) higher at 24 h post-ingestion Vitamin E. The pattern of response was not significantly different whether Vitamin C (or Vitamin E) was taken alone or in combination, indicating no augmentation of response to one by co-ingestion of the other vitamin. No significant changes were seen in plasma FRAP in the group overall (although increases (P < 0.05) were seen at 90 and 180 min post-ingestion in women after Vitamin C ingestion) or in MDA across treatments, and no evidence of increased DNA damage, or of DNA protection, was seen at any time point after Vitamin C and/or E ingestion. In conclusion, the data from this first controlled study of acute effects of single doses of Vitamin C and/or E show no evidence of either a protective or deleterious effect on DNA damage, resistance of DNA to oxidant challenge, or lipid peroxidation. No evidence of a synergistic or cooperative interaction between Vitamins C and E was seen, but further study is needed to determine possible interactive effects in a staggered supplementation cycle, and study of subjects under increased oxidative stress or with marginal antioxidant status would be useful. It would be of interest also to study the effects of these vitamins ingested with, or in, whole food, to determine if they are directly protective at doses above the minimum required to prevent deficiency, if combinations with other food components are needed for effective protection, or if Vitamins C and E are largely surrogate biomarkers of a 'healthy' diet, but are not the key protective agents. (C) 2004 Elsevier B.V. All rights reserved.