Picobirnaviruses encode a protein with repeats of the ExxRxNxxxE motif
Picobirnaviruses encode a protein with repeats of the ExxRxNxxxE motif
复制标题
DOI：
10.1016/j.virusres.2011.02.018
复制
发表时间：
2011-06-01
期刊：
影响因子：
5
通讯作者：
Delmas, Bernard
中科院分区：
文献类型：
--
作者：
Da Costa, Bruno;Duquerroy, Stephane;Delmas, Bernard
Picobirnaviruses具有双节段双链RNA基因组。片段2编码RNA依赖性RNA聚合酶，而片段1显示两个开放阅读框（ORF）。ORF 2最近被证明编码衣壳前体，而ORF 1产物尚未被表征。在这项研究中，我们表明，数据库中的三个ORF 1序列，代表三个遗传学上遥远的picobirnaviruses（两个来自人类，一个来自兔宿主）编码不同大小的蛋白质（106-224个残基，不含脯氨酸和半胱氨酸），根据病毒种类，这些蛋白质含有一个特定的序列基序（ExxRxNxxxE），重复4到10次。几种算法预测这三种蛋白质主要在含有重复序列的结构域中展开。富含甘氨酸的25-40个氨基酸长的C-末端结构域包含具有3-4个残基的周期性的疏水残基，预测其在结构上不同于包含基序重复的上游结构域。ExxRxNxxxE序列先前未被鉴定为真核和原核蛋白质中的短线性基序。它的功能仍然难以捉摸。(C)2011爱思唯尔有限公司版权所有。
Picobirnaviruses possess a bisegmented double-stranded RNA genome. While the segment 2 encodes the RNA-dependent RNA polymerase, the segment 1 displays two open reading frames (ORFs). ORF2 was recently shown to code the capsid precursor and ORF1 product has not been characterized. In this study, we show that the three ORF1 sequences available in databases and representing three phylogenetically distant picobirnaviruses (two from human and one from rabbit hosts) encode proteins of various sizes (106-224 residues and without proline and cysteine) harbouring a particular sequence motif (ExxRxNxxxE) repeated four to ten times, depending on the virus species. Several algorithms predicted the three proteins to be mainly unfolded in the domains containing the repeats. The glycine-rich 25-40 amino acid long C-terminal domains containing hydrophobic residues with a periodicity of 3-4 residues are predicted structurally different of the upstream domains containing the motif repetitions. The ExxRxNxxxE sequence was not previously identified as a short linear motif in eukaryotic and prokaryotic proteins. Its function remains elusive. (C) 2011 Elsevier B.V. All rights reserved.