NOA1 functions in a temperature-dependent manner to regulate chlorophyll biosynthesis and Rubisco formation in rice.
NOA1 functions in a temperature-dependent manner to regulate chlorophyll biosynthesis and Rubisco formation in rice.
复制标题
DOI：
10.1371/journal.pone.0020015
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Peng X
中科院分区：
文献类型：
--
作者：
Yang Q;He H;Li H;Tian H;Zhang J;Zhai L;Chen J;Wu H;Yi G;He ZH;Peng X
NOA1编码一种环状排列的GT3（cGT3），是植物核糖体组装所必需的。虽然以前在NOA1抑制的水稻和拟南芥中注意到叶绿素和Rubisco表型的减少，但仍然需要详细的了解。在本研究中，通过使用RNAi转基因水稻，我们进一步证明了NOA1的功能，在温度依赖性的方式来调节叶绿素和Rubisco水平。当植物在30°C下生长时，OsNOA1沉默的植物中的叶绿素和Rubisco水平仅略低于WT中的那些。然而，在22°C下，沉默的植物积累的叶绿素和Rubisco远低于WT。进一步揭示了叶绿素和Rubisco的调节发生在合成代谢水平。黄化的WT幼苗一旦在30°C或15°C下恢复光照，就容易恢复叶绿素和Rubisco积累。黄化的OsNOA1沉默植株仅在30°C下才能积累叶绿素和Rubisco，而在低温下则失去了这种能力。另一方面，去黄化的OsNOA1沉默的幼苗保持与WT相似的叶绿素和Rubisco水平，即使在被转移到15°C不同时间之后。进一步的表达分析确定了几个候选基因，包括OsPorA（NADPH：原叶绿素氧化还原酶A）、OsrbcL（Rubisco大亚基）、OsRALyase（核糖体RNA脱嘌呤位点特异性裂解酶）和OsPuf 4（Puf家族的RNA结合蛋白），它们可能参与OsNOA1调节的叶绿素生物合成和Rubisco形成。总之，我们的研究结果表明，OsNOA1功能的温度依赖性的方式来调节水稻叶绿素合成，Rubisco的形成和质体发育。
NITRIC OXIDE-ASSOCIATED1 (NOA1) encodes a circularly permuted GTPase (cGTPase) known to be essential for ribosome assembly in plants. While the reduced chlorophyll and Rubisco phenotypes were formerly noticed in both NOA1-supressed rice and Arabidopsis, a detailed insight is still necessary. In this study, by using RNAi transgenic rice, we further demonstrate that NOA1 functions in a temperature-dependent manner to regulate chlorophyll and Rubisco levels. When plants were grown at 30°C, the chlorophyll and Rubisco levels in OsNOA1-silenced plants were only slightly lower than those in WT. However, at 22°C, the silenced plants accumulated far less chlorophyll and Rubisco than WT. It was further revealed that the regulation of chlorophyll and Rubisco occurs at the anabolic level. Etiolated WT seedlings restored chlorophyll and Rubisco accumulations readily once returned to light, at either 30°C or 15°C. Etiolated OsNOA1-silenced plants accumulated chlorophyll and Rubisco to normal levels only at 30°C, and lost this ability at low temperature. On the other hand, de-etiolated OsNOA1-silenced seedlings maintained similar levels of chlorophyll and Rubisco as WT, even after being shifted to 15°C for various times. Further expression analyses identified several candidate genes, including OsPorA (NADPH: protochlorophyllide oxidoreductase A), OsrbcL (Rubisco large subunit), OsRALyase (Ribosomal RNA apurinic site specific lyase) and OsPuf4 (RNA-binding protein of the Puf family), which may be involved in OsNOA1-regulated chlorophyll biosynthesis and Rubisco formation. Overall, our results suggest OsNOA1 functions in a temperature-dependent manner to regulate chlorophyll biosynthesis, Rubisco formation and plastid development in rice.
登录
查看更多内容
影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
影响因子：
7.2
作者：
Bright, J;Desikan, R;Neill, SJ
通讯作者：
Neill, SJ
影响因子：
4.8
作者：
Moreau, Magali;Lee, Gyu In;Klessig, Daniel F.
通讯作者：
Klessig, Daniel F.
一氧化氮在黄瓜油菜素类固醇过氧化氢依赖性诱导非生物胁迫耐受性中的作用
影响因子：
7.3
作者：
Cui, Jin-Xia;Zhou, Yan-Hong;Yu, Jing-Quan
通讯作者：
Yu, Jing-Quan
影响因子：
7.4
作者：
BEVINS, MA;MADHAVAN, S;MARKWELL, J
通讯作者：
MARKWELL, J