Caspase-3 activity as a prognostic factor in colorectal carcinoma
Caspase-3 activity as a prognostic factor in colorectal carcinoma
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DOI：
10.1038/labinvest.3780277
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发表时间：
2001-05-01
影响因子：
5
通讯作者：
Kuppen, PJK
中科院分区：
文献类型：
--
作者：
Jonges, LE;Nagelkerke, JF;Kuppen, PJK
已经描述了几种确定肿瘤中凋亡频率的技术。在这项研究中，我们报告，生化检测酶caspase-3活性是一种简单的和定量的技术来衡量大肠癌细胞凋亡。结直肠癌细胞凋亡水平与临床病程的相关性尚不清楚。因此，我们研究了caspase-3活性和疾病预后之间的相关性，已知参与细胞凋亡诱导的不同因素。高caspase-3活性与较高的复发风险显著相关，并且优先见于右侧的肿瘤。结肠高caspase-3活性与p53、β-连环蛋白或错配修复基因蛋白质表达改变之间没有相关性。这表明高caspase-3活性与遗传Wnt信号传导或错配修复突变途径没有明显的相关性。caspase-3活性与CD 57（+）肿瘤浸润细胞数显著相关。因此，右侧肿瘤中的高caspase-3活性可能由特异性淋巴细胞反应诱导。
Several techniques to determine apoptotic frequencies in tumors have been described. In this study, we report that biochemical detection of enzymatic caspase-3 activity is a simple and quantitative technique to measure apoptosis in colorectal tumor cells. The relevance of the level of apoptosis in colorectal cancer for the clinical course remains unclear. Therefore, we studied the correlation between caspase-3 activity and prognosis of the disease in relation to different factors known to be involved in apoptosis induction. High caspase-3 activity significantly correlated with a higher risk of recurrence and was preferentially found in tumors of the right side of the! colon. No correlation was detected between high caspase-3 activity and altered protein expression of p53, beta -catenin, or proteins of mismatched repair genes. This indicates that high caspase-3 activity has no evident correlation with the genetic Wnt-signaling or the mismatch repair mutational pathways. The caspase-3 activity significantly correlated with CD57(+) tumor infiltrating cells. Therefore, high caspase-3 activity in right-sided tumors might be induced by a specific lymphocytic reaction.