Measuring Daphnia life history in the wild: The efficacy of individual field cages.
Measuring Daphnia life history in the wild: The efficacy of individual field cages.
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DOI：
10.1002/ece3.8326
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发表时间：
2021-12
影响因子：
2.6
通讯作者：
Plaistow SJ
中科院分区：
文献类型：
--
作者：
O'Connor M;Sadler DE;Brunner FS;Reynolds A;White N;Price S;Plaistow SJ
生活史研究通常是在实验室环境中进行的，在实验室环境中很容易对单个动物进行分析。然而，温度、光周期和营养等因素在实验室和田间环境之间差异很大，因此很难对结果进行比较。因此，有必要研究该领域的个体生活史，但目前在水蚤等系统中，这是困难的，因为不可能标记和跟踪个体动物。在这里，我们提出了一项原理证明研究，表明野外笼是收集大水蚤个体水平生活史数据的可靠方法。作为第一步，我们比较了笼子内外饲养的成对动物的生活史，以验证笼子允许藻类食物资源自由流动的假设。然后，我们使用了一种半自然的介生态环境来比较单独的野外笼和每天装满介生态水的玻璃罐的性能。我们发现笼子不会抑制食物流动，并且在罐子试验中检测到的三个克隆之间的生活史差异也可以在使用劳动强度低得多的野外笼子时检测到。我们得出的结论是，野外笼是一种可行的方法，可以在水蚤等动物个体无法被标记和跟踪的系统中收集个体水平的生活史数据。有必要研究该领域的个体生活史，但目前在水蚤等系统中，这是困难的，因为不可能标记和跟踪个体动物。在这里，我们验证了野外笼是收集大水蚤个体水平生活史数据的有效方法的假设。我们提供的证据表明，我们的笼子设计允许在自然环境中收集真实、可靠和可重复的生活史数据，因为笼子里的动物体验环境的方式与笼子外面的动物相同。
Life‐history studies are often conducted in a laboratory environment where it is easy to assay individual animals. However, factors such as temperature, photoperiod, and nutrition vary greatly between laboratory and field environments, making it difficult to compare results. Consequently, there is a need to study individual life histories in the field, but this is currently difficult in systems such as Daphnia where it is not possible to mark and track individual animals. Here, we present a proof of principle study showing that field cages are a reliable method for collecting individual‐level life‐history data in Daphnia magna. As a first step, we compared the life history of paired animals reared outside and inside cages to test the hypothesis that cages allow free flow of algal food resources. We then used a seminatural mesocosm setting to compare the performance of individual field cages versus glass jars refilled with mesocosm water each day. We found that cages did not inhibit food flow and that differences in life histories between three clones detected in the jar assays were also detectable using the much less labor‐intensive field cages. We conclude that field cages are a feasible approach for collecting individual‐level life‐history data in systems such as Daphnia where individual animals cannot be marked and tracked. There is a need to study individual life histories in the field, but this is currently difficult in systems such as Daphnia where it is not possible to mark and track individual animals. Here, we test the hypothesis that field cages are a useful method for collecting individual‐level life‐history data in Daphnia magna. We provide evidence that our cage design allows the collection of realistic, reliable, and repeatable measurements of life‐history data in the natural setting, as animals inside the cages experience the environment in the same way as those outside.
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影响因子：
2.7
作者：
Lagerspetz, KYH
通讯作者：
Lagerspetz, KYH
影响因子：
2.1
作者：
Hammill, E.;Rogers, A.;Beckerman, A. P.
通讯作者：
Beckerman, A. P.
影响因子：
2.7
作者：
Giebelhausen, B;Lampert, W
通讯作者：
Lampert, W
影响因子：
2.7
作者：
KORPELAINEN, H
通讯作者：
KORPELAINEN, H
影响因子：
2
作者：
Grodwohl, Jean-Baptiste;Porto, Franco;El-Hani, Charbel N.
通讯作者：
El-Hani, Charbel N.