Evaluation of pretreatment with Pleurotus ostreatus for enzymatic hydrolysis of rice straw
Evaluation of pretreatment with Pleurotus ostreatus for enzymatic hydrolysis of rice straw
复制标题
DOI：
10.1263/jbb.100.637
复制
发表时间：
2005-12-01
影响因子：
2.8
通讯作者：
Tanaka, T
中科院分区：
文献类型：
--
作者：
Taniguchi, M;Suzuki, H;Tanaka, T
研究了黄孢原毛平革菌(Phanerochaete Chrysosporium)、杂色毛霉(Trametes Versicolor)、枯草芽孢霉(Ceriporiopsis Subvermispora)和平菇(Pleurotus Ostreatus)4种白腐菌对稻草的生物预处理效果。在这些白腐菌中，平菇选择性地降解稻草中的木质素部分，而不是综纤维素成分。稻草(含水率为60%)经平菇处理60d后，总失重率为25%，克拉森木质素降解率为41%。处理后稻草中的纤维素和半纤维素残留量分别是未处理稻草的83%和52%。用商品化纤维素酶制剂48111对稻草进行酶解，可使稻草中52%的综纤维素和44%的纤维素溶解。以综纤维素和纤维素量计算，未处理稻草的总糖产量为33%，葡萄糖产量为32%。扫描电子显微镜观察表明，稻草经平菇处理后，其对酶解敏感性的增加是由于木质素的部分降解所致。当Klason木质素含量低于处理后秸秆总质量的15%时，随着Klason木质素含量的降低，综纤维素和纤维素组分的酶促增溶程度增强。
The effects of biological pretreatment of rice straw using four white-rot fungi (Phanerochaete chrysosporium, Trametes versicolor, Ceriporiopsis subvermispora, and Pleurotus ostreatus) were evaluated on the basis of quantitative and structural changes in the components of the pretreated rice straw as well as susceptibility to enzymatic hydrolysis. Of these white-rot fungi, P ostreatus selectively degraded the lignin fraction of rice straw rather than the holocellulose component. When rice straw (water content of 60%) was pretreated with P ostreatus for 60 d, the total weight loss and the degree of Klason lignin degraded were 25% and 41%, respectively. After the pretreatment, the residual amounts of cellulose and hemicellulose were 83% and 52% of those in untreated rice straw, respectively. By enzymatic hydrolysis with a commercial cellulase preparation for 48 111, 52% holocellulose and 44% cellulose in the pretreated rice straw were solubilized. The net sugar yields based on the amounts of holocellulose and cellulose of untreated rice straw were 33% for total soluble sugar from holocellulose and 32% for glucose from cellulose. The SEM observations showed that the increase in susceptibility of rice straw to enzymatic hydrolysis by pretreatment with P ostreatus is caused by partial degradation of the lignin seal. When the content of Klason lignin was less than 15% of the total weight of the pretreated straw, enhanced degrees of enzymatic solubilization of holocellulose and cellulose fractions were observed as the content of Klason lignin decreased.