Flavonoids in Selected Mediterranean Fruits: Extraction, Electrochemical Detection and Total Antioxidant Capacity Evaluation
Flavonoids in Selected Mediterranean Fruits: Extraction, Electrochemical Detection and Total Antioxidant Capacity Evaluation
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DOI：
10.1002/elan.201600370
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发表时间：
2017-02-01
期刊：
影响因子：
3
通讯作者：
Oliveira-Brett, Ana Maria
中科院分区：
文献类型：
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作者：
Issaad, Fatima Zohra;Fernandes, Isabel P. G.;Oliveira-Brett, Ana Maria
黄酮类化合物是天然酚类衍生物，其在低浓度下可以通过防止生物分子（蛋白质、核酸、脂质、糖）经由自由基介导的反应的氧化损伤来提供健康益处。微波-超声辅助提取后，采用反相高效液相色谱法，结合光电二极管阵列和电化学检测器，对选定的地中海时令水果：杏、酸樱桃、李子、石榴、枣子、仙人掌果和油桃中的黄酮类化合物进行了检测。使用黄酮标准品。采用微分脉冲伏安法（电化学指数-EI）、积分峰面积和计时电流法对冻干水果提取物的总抗氧化能力进行了评价。在冻干的水果提取物中，以儿茶素为标准品，自由基清除剂有效浓度（EC 50），采用DPPH。测定，确定。
Flavonoids are natural phenolic derivatives that, in low concentration, can provide health benefits by preventing biomolecules (proteins, nucleic acids, lipids, sugars) oxidative damage through free radical mediated reactions. The flavonoids, in selected Mediterranean seasonal fruits: apricot, sour cherry, plum, pomegranate, date, prickly pear (cactus fruit), and nectarine, by RP-HPLC, coupled with photodiode array and electrochemical detectors, after microwave-ultrasound assisted extraction, using flavonoid standards, were detected. The total antioxidant capacity in the lyophilized fruit extracts, by differential pulse voltammetry, (electrochemical index-EI), integrated peak area, and chronoamperometry, was evaluated. In the lyophilized fruit extracts, and the catechin standard, the free radical scavenger efficient concentration (EC50), using DPPH. assay, was determined.