Connections of Barrington's nucleus to the sympathetic nervous system in rats
Connections of Barrington's nucleus to the sympathetic nervous system in rats
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DOI：
10.1016/s0165-1838(99)00101-0
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发表时间：
2000-03-15
期刊：
影响因子：
--
通讯作者：
Sved, AF
中科院分区：
文献类型：
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作者：
Cano, G;Card, JP;Sved, AF
巴灵顿核 (BN) 被认为是专门与骨盆副交感神经活动控制相关的脑桥中心。本研究证明了 BN 和自主神经流出到仅由自主神经系统交感神经支配的内脏目标之间的解剖学联系。将伪狂犬病病毒（PRV）（一种逆行突触示踪剂）注射到两个交感神经支配的器官（脾和肾）后的感染时间分析显示，在接种后早期存活时间间隔中，BN 中存在受感染的神经元。脾脏注射后 PRV 的免疫组织化学定位显示，一小部分 BN 神经元在与已知投射到胸脊髓交感节前神经元 (SPN) 的其他大脑区域中受感染神经元的出现一致的时间范围内被标记；在将 PRV 注射到脾或肾后，在 BN 中每隔一段时间就会出现大量受感染的神经元。逆行示踪剂氟金腹膜内共注射PRV进入脾脏表明尾髓或腰骶脊髓中的副交感神经节前神经元没有被感染，这表明受感染的BN神经元不是通过副交感神经途径感染的。因此，在 PRV 注射到脾或肾后，BN 神经元直接通过 BN 投射到 SPN 或其次通过 BN 投射到感染的节前神经元而被感染。这些结果证明了 BN 和交感神经活动之间存在直接或间接的解剖学联系。由于 BN 接收来自不同大脑区域的大量输入，因此 BN 与自主神经系统两个分支的关系表明，该核可能在与交感神经和副交感神经活动的中枢协调相关的脊髓上输入整合中发挥作用。 (C) 2000 年由 Elsevier Science B.V. 出版。保留所有权利。
Barrington's nucleus (BN) has been considered a pontine center related exclusively to the control of pelvic parasympathetic activity. The present study demonstrates an anatomical linkage between BN and autonomic outflow to visceral targets innervated exclusively by the sympathetic division of the autonomic nervous system. Temporal analysis of infection after injection of pseudorabies virus (PRV), a retrograde transynaptic tracer, into two sympathetically innervated organs, the spleen and the kidney, revealed the presence of infected neurons in BN at early post-inoculation survival intervals. Immunohistochemical localization of PRV after spleen injections showed that a small subpopulation of BN neurons became labeled in a time frame coincident with the appearance of infected neurons in other brain regions known to project to sympathetic preganglionic neurons (SPNs) in the thoracic spinal cord; a larger number of infected neurons appeared in BN at intermediate intervals after PRV injections into the spleen or kidney. Coinjection of the retrograde tracer Fluoro-Gold i.p. and PRV into the spleen demonstrated that parasympathetic preganglionic neurons in the caudal medulla or lumbo-sacral spinal cord were not infected, indicating that infected BN neurons were not infected via a parasympathetic route. Thus, BN neurons become infected after PRV injections into the spleen or kidney either directly through BN projections to SPNs, or secondarily via BN projections to infected pre-preganglionic neurons. These results demonstrate an anatomical linkage, either direct or indirect, between BN and sympathetic activity. Because BN receives numerous inputs from diverse brain regions, the relation of BN with both branches of the autonomic nervous system suggests that this nucleus might play a role in the integration of supraspinal inputs relevant to the central coordination of sympathetic and parasympathetic activity. (C) 2000 Published by Elsevier Science B.V. All rights reserved.