Polymerisation of lignins by ligninases from Phanerochaete chrysosporium
Polymerisation of lignins by ligninases from Phanerochaete chrysosporium
复制标题
来自黄孢原毛平革菌的木质素酶对木质素的聚合
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
A. Fiechter
中科院分区：
文献类型：
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作者：
S. Haemmerli;M. Leisola;A. Fiechter
白腐真菌黄孢原毛平革菌(Phanerochaete Chrysosporium)在碳限制条件下生产的胞外粗酶制剂经等电聚焦纯化得到四种主要的血红蛋白。在过氧化氢存在下，粗酶和纯化的蛋白质均能氧化磨木素、盐酸二氧六环提取的秸秆木质素和碱秸秆木质素。氧化主要导致木质素的进一步聚合，并通过向反应混合物中添加藜芦醇来增强。碱秸秆木质素也能被辣根过氧化物酶聚合，但藜芦醇对该反应没有影响。
Four major hemoproteins were purified by isoelectric focusing from an extracellular crude enzyme preparation, produced by the white rot fungus Phanerochaete chrysosporium under carbon-limited conditions. Both the crude enzyme and the purified proteins oxidised milled wood lignin, HCl-dioxane-extracted straw lignin and alkali straw lignin in the presence of hydrogen peroxide. The oxidation resulted mainly in further polymerisation of the lignins and was enhanced by addition of veratryl alcohol to the reaction mixture. Alkali straw lignin was also polymerised by horseradish peroxidase, although veratryl alcohol had no influence on this reaction.
Phanerochaete chrysosporium 木质素酶对木质素模型二聚体进行氧化 C α-C β 裂解的机制。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Hammel,KE;Tien,M;Kalyanaraman,B;Kirk,TK
通讯作者：
Kirk,TK