Alterations in cerebral metabolomics and proteomic expression during sepsis
Alterations in cerebral metabolomics and proteomic expression during sepsis
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DOI：
10.2174/156720207782446388
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发表时间：
2007-11-01
影响因子：
2.1
通讯作者：
Kalenka, Armin
中科院分区：
文献类型：
--
作者：
Hinkelbein, Jochen;Feldmann, Robert E.;Kalenka, Armin
脓毒症和感染性脑病期间脑功能障碍的原因仍在研究中。脓毒症引起的脑蛋白表达变化可能对脓毒性脑病的认识起重要作用。本研究的目的是探讨脓毒症大鼠大脑蛋白质组的改变。56只Wistar交配大鼠随机分为脓毒症组（结扎和穿刺，CLP）和对照组（假手术）。手术后24或48小时杀死存活的大鼠，全脑裂解液用于二维凝胶电泳和随后的蛋白质鉴定。质谱法鉴定差异表达蛋白。利用匠心途径分析（Ingenuity Pathways Analysis， IPA）工具，分析鉴定蛋白之间的关系和相互作用。脓毒症大鼠的死亡率为53%。对照组无大鼠丢失。每个凝胶有1100多个点被区分出来，其中29种不同的蛋白质显著(2倍，P
The cause of brain dysfunction during sepsis and septic encephalopathy is still under ongoing research. Sepsis induced changes in cerebral protein expression may play a significant role in the understanding of septic encephalopathy. The aim of the present study was to explore cerebral proteome alterations in septic rats. Fifty-six mate Wistar rats were randomly assigned to a sepsis group (coecal ligature and puncture, CLP) or a control group (sham). Surviving rats were killed 24 or 48 hours after surgery and whole-brain lysates were used for two-dimensional gel electrophoresis and subsequent protein identification. Differentially expressed proteins were identified by mass spectrometry. Using the Ingenuity Pathways Analysis (IPA) tool, the relationship and interaction between the identified proteins was analyzed. Mortality was 53 % in septic rats. No rat of the control group was lost. More than 1,100 spots per gel were discriminated of which 29 different proteins were significantly (2-fold, P