Staphylococcus aureus and MRSA Growth and Biofilm Formation after Treatment with Antibiotics and SeNPs.
Staphylococcus aureus and MRSA Growth and Biofilm Formation after Treatment with Antibiotics and SeNPs.
复制标题
DOI：
10.3390/ijms161024656
复制
发表时间：
2015-10-16
影响因子：
5.6
通讯作者：
Kizek R
中科院分区：
文献类型：
--
作者：
Cihalova K;Chudobova D;Michalek P;Moulick A;Guran R;Kopel P;Adam V;Kizek R
耐甲氧西林金黄色葡萄球菌（MRSA）是一种对β-内酰胺类抗生素耐药的危险病原菌。由于其耐药性，很难控制该菌株引起的感染。我们研究了这个问题，在敏感的S。金黄色葡萄球菌和MRSA后应用抗生素（ATB）-氨苄青霉素，苯唑西林和青霉素-和硒纳米颗粒（SeNPs）与这些ATB的复合物。结果表明SeNPs与常规ATB的复合物具有较强的抑制作用。使用阻抗法，与暴露于不含SeNP的ATB的组相比，在应用ATB与SeNP的复合物后观察到生物膜的更高破坏。生物膜的形成受到强烈的抑制（对S.金黄色葡萄球菌和高达94% ± 4%的MRSA），而不含SeNP的ATB抑制S.金黄色葡萄球菌高达79% ± 5%，MRSA仅高达16% ± 2%。所获得的结果为使用SeNP作为治疗细菌感染的工具提供了基础，由于细菌对常规ATB药物的耐药性增加，这可能是复杂的。
Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to β-lactam antibiotics. Due to its resistance, it is difficult to manage the infections caused by this strain. We examined this issue in terms of observation of the growth properties and ability to form biofilms in sensitive S. aureus and MRSA after the application of antibiotics (ATBs)—ampicillin, oxacillin and penicillin—and complexes of selenium nanoparticles (SeNPs) with these ATBs. The results suggest the strong inhibition effect of SeNPs in complexes with conventional ATBs. Using the impedance method, a higher disruption of biofilms was observed after the application of ATB complexes with SeNPs compared to the group exposed to ATBs without SeNPs. The biofilm formation was intensely inhibited (up to 99% ± 7% for S. aureus and up to 94% ± 4% for MRSA) after application of SeNPs in comparison with bacteria without antibacterial compounds whereas ATBs without SeNPs inhibited S. aureus up to 79% ± 5% and MRSA up to 16% ± 2% only. The obtained results provide a basis for the use of SeNPs as a tool for the treatment of bacterial infections, which can be complicated because of increasing resistance of bacteria to conventional ATB drugs.