Application of reflected light microscopy to identify and quantitate resorption by isolated osteoclasts
Application of reflected light microscopy to identify and quantitate resorption by isolated osteoclasts
复制标题
应用反射光显微镜识别和定量分离破骨细胞的吸收
DOI：
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发表时间：
1991
影响因子：
6.2
通讯作者：
J. Gallagher
中科院分区：
文献类型：
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作者：
C. A. Walsh;J. Beresford;M. Birch;B. Boothroyd;J. Gallagher
一种简单的技术已被开发出来，以确定骨吸收陷窝挖破骨细胞在体外。简单地说，将具有原位细胞的灭活牛骨薄片固定，用甲苯胺蓝染色，然后通过反射光显微镜检查。使用x 10物镜，即使吸收区域被细胞融合层覆盖，也能清晰地看到吸收陷窝。该技术可用于量化开挖的平面面积、深度和体积。使用x 50物镜透镜评估深度和体积。该技术已被用于研究醋酸视黄酯和EHDP对骨吸收的影响。
A simple technique has been developed to identify the resorption lacunae excavated by avian osteoclasts in vitro. Briefly, devitalized bovine bone wafers, with cells in situ, are fixed, stained with toluidine blue, and then examined by reflected light microscopy. Resorption pits are clearly visible using a x 10 objective, even when the resorbed areas are covered by a confluent layer of cells. The technique can be used to quantify the plan area, depth, and volume of the excavations. Depth and volume are assessed using the x 50 objective lens. This technique has been used to investigate the effects of retinyl acetate and EHDP on bone resorption.