Lentinan-Functionalized Graphene Oxide Is an Effective Antigen Delivery System That Modulates Innate Immunity and Improves Adaptive Immunity
Lentinan-Functionalized Graphene Oxide Is an Effective Antigen Delivery System That Modulates Innate Immunity and Improves Adaptive Immunity
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香菇多糖功能化氧化石墨烯是一种有效的抗原传递系统，可调节先天免疫并提高适应性免疫。
DOI：
10.1021/acsami.0c12078
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发表时间：
2020-09-02
影响因子：
9.5
通讯作者：
Wang, Deyun
中科院分区：
文献类型：
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作者：
Liu, Zhenguang;He, Jin;Wang, Deyun
氧化石墨烯(GO)和香菇多糖因其在生物医学中的应用而备受关注。氧化石墨烯因其生物相容性、较大的比表面积和优异的吸附能力而被用于药物和疫苗输送系统，而香菇多糖则具有增强免疫的作用。在本研究中，我们合成并表征了接枝LNT作为佐剂的GO，并研究了其对免疫应答的影响。香菇多糖修饰的GO(GO-LNT)可促进巨噬细胞摄取抗原，提高体外抗原应用效率。此外，在体内，与GO/OVA相比，GO-LNT/OVA可降低卵清蛋白的释放率以维持长期免疫应答，并提高免疫球蛋白和免疫球蛋白S亚型的水平。因此，我们可以推断，GO-LNT的作用是由于细胞摄取抗原的数量增加所致。总体而言，我们的研究表明，GO-LNT可以满足安全有效的疫苗递送系统以及一种优秀的佐剂，既能引起长期的免疫记忆反应，又能增强细胞和体液免疫。
The graphene oxide (GO) and lentinan have received great attention because of their utility in biomedical applications. Graphene oxide is utilized in drug and vaccine delivery systems due to its biocompatibility, large surface area, and outstanding adsorption capability, while lentinan has immunity-enhancing effects. In this study, we synthesized and characterized GO grafted with LNT as an adjuvant, and investigated how to impact the immune responses. Lentinan-modified GO (GO-LNT) facilitated antigen uptake in macrophages and improved the efficiency of antigen application in vitro. Furthermore, in vivo, compared with GO/OVA, GO-LNT/OVA decreased the release rate of OVA to sustain long-term immune responses, and boost the levels of IgG and IgG s subtypes. Hence, we can infer that the effects of GO-LNT were a result of the increased amounts of antigen uptake by cells. Overall, our studies demonstrated that GO-LNT could suffice for a safe and effective vaccine delivery system as well as an excellent adjuvant that both elicits a long-term immune memory response and potentiates cellular and humoral immunity.