SYNTHESIS AND UTILITY OF 5-THIOCYANATO DEOXYURIDINE AND URIDINE PHOSPHORAMIDITES AS MASKED SYNTHONS
SYNTHESIS AND UTILITY OF 5-THIOCYANATO DEOXYURIDINE AND URIDINE PHOSPHORAMIDITES AS MASKED SYNTHONS
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DOI：
10.1016/s0040-4039(00)60938-3
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发表时间：
1992-10-13
影响因子：
1.8
通讯作者：
HANNA, MM
中科院分区：
文献类型：
--
作者：
BRADLEY, DH;HANNA, MM
合成了5-硫氰酸基-2-脱氧尿苷和5-硫氰酸尿苷亚磷酰胺，并通过自动化方法将其引入到DNA和RNA三聚体中。用DTT处理去除了SCN基团，并通过生成的硫醇基团将光交联芳基叠氮化物添加到DNA三核苷酸中。
5-Thiocyanato-2-deoxyuridine and 5-thiocyanato-uridine phosphoramidites have been synthesized and incorporated into DNA and RNA trimers via automated methodology. The SCN group has been removed with DTT treatment, and a photocrosslinking aryl azide has been added to the DNA trinucleotide through the resulting thiol group.