Distribution of antibiotic resistance and the presence of vancomycin-resistance genes (vanA and vanB) in Enterobacteriaceae isolated from the Sea of Marmara, the Canakkale Strait and the Istanbul Strait, Turkey
Distribution of antibiotic resistance and the presence of vancomycin-resistance genes (vanA and vanB) in Enterobacteriaceae isolated from the Sea of Marmara, the Canakkale Strait and the Istanbul Strait, Turkey
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DOI：
10.1515/ohs-2016-0017
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发表时间：
2016-06-01
影响因子：
0.9
通讯作者：
Erol, Ozlem
中科院分区：
文献类型：
--
作者：
Cardak, Mine;Altug, Gulsen;Erol, Ozlem
我们调查了从土耳其马尔马拉海、伊斯坦布尔和恰纳卡莱海峡采集的样本中肠杆菌科细菌的抗生素耐药性频率以及万古霉素耐药基因的存在情况。使用 VITEK 2 Compact 30 系统分离并鉴定不同的菌落形成细菌。采用纸片扩散法测定分离株的抗生素耐药性。对分离株进行了抗阿莫西林、氨苄西林、氨曲南、头孢他啶、头孢噻肟、头孢呋辛、氧氟沙星、万古霉素、四环素、卡那霉素和庆大霉素的测试。还研究了万古霉素抗性基因（vanA 和 vanB）的存在。马尔马拉海肠杆菌属的水平高于伊斯坦布尔海峡和恰纳卡莱海峡。从大肠杆菌分离株中鉴定出对最多抗生素具有抗性的分离株。所选菌株的耐药性如下：卡那霉素（82%）、万古霉素（78%）和氨苄西林（60%）。一些对万古霉素具有中等耐药性的肠杆菌科细菌分离株具有 vanA 基因。这项研究提供了海洋环境中细菌对临床相关抗生素广泛耐药的证据。它还有助于了解肠杆菌科抗生素耐药性的分布，并表明控制措施在生活水处理中的重要性。
We investigated the frequency of antibiotic resistance of Enterobacteriaceae and the presence of vancomyc-in-resistance genes in samples taken from the Sea of Marmara, and the Istanbul and Canakkale Straits, Turkey. Different colony -forming bacteria were isolated and identified with the VITEK 2 Compact 30 system. The antibiotic resistance of the isolates was determined by the disc diffusion method. The isolates were tested against amoxicilin, ampicillin, aztreonam, ceftazidime, cefotaxime, cefuroxime, ofloxacin, vancomycin, tetracycline, kanamycin and gentamycin. The presence of vancomyctn-resistance genes (vanA and vanB) was also investigated. The level of Enterobucteriaceue species was higher in the Sea of Marmara than in the Istanbul Strait and the Canakkale Strait. Isolates showing resistance to the greatest number of antibiotics were identified from E. colt isolates. The resistance of the selected bacterial isolates were as follows: kanamycin (82%), vancomycin (78%) and ampicillin (60%). Some intermediately vancomycin-resistant Enterobacteriaceae isolates had the vanA gene. This study provides evidence of widespread bacterial resistance to clinically relevant antibiotics in marine environments. It also contributes to the knowledge on the distribution of antibiotic resistance among Enterobacteriaceae and indicates the importance of control measures in domestic water treatment.