Identification of seven metabolites of oxyresveratrol in rat urine and bile using liquid chromatography/tandem mass spectrometry
Identification of seven metabolites of oxyresveratrol in rat urine and bile using liquid chromatography/tandem mass spectrometry
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液相色谱/串联质谱法鉴定大鼠尿液和胆汁中氧化白藜芦醇的七种代谢物
DOI：
10.1002/bmc.1309
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发表时间：
2010-04-01
影响因子：
1.8
通讯作者：
Guo, De-An
中科院分区：
文献类型：
--
作者：
Huang, Huilian;Chen, Guangtong;Guo, De-An
氧化白藜芦醇(反式-2，4，3‘，5’-四羟基二苯乙烯)是从中草药中国中分离得到的主要化合物。大鼠口服氧化白藜芦醇100 mg/kg后，用固相萃取法对尿液和胆汁样品进行前处理。采用负离子模式电喷雾电离LC-MS/MS方法对7种代谢物进行了鉴定。结果表明，氧化白藜芦醇的主要代谢产物为单葡萄糖醛酸化和单硫化的氧化白藜芦醇。在此基础上，提出了氧化白藜芦醇在大鼠尿和胆汁中的代谢途径。版权所有(C)2009 John Wiley&Sons，Ltd.
Oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene) is a major compound isolated from Smilax china, a Chinese herbal medicine. The rat urine and bile samples were pretreated by solid-phase extraction method after oral administration at a dose of 100 mg/kg of oxyresveratrol. Seven metabolites were identified by LC-MS/MS method with electrospray ionization in negative ion mode. The results indicated that main metabolites of oxyresveratrol were monoglucuronided and monosulfated oxyresveratrol. Based on the results, the metabolic pathway of oxyresveratrol in rat urine and bile was proposed. Copyright (C) 2009 John Wiley & Sons, Ltd.